Cort N, Fredriksson G, Kindahl H, Edqvist L E, Rylander R
Department of Obstetrics and Gynaecology, Faculty of Veterinary Medicine, Swedish University of Agricultural Sciences, Uppsala, Sweden.
Zentralbl Veterinarmed A. 1990 Mar;37(2):130-7. doi: 10.1111/j.1439-0442.1990.tb00884.x.
Endotoxin of Gram-negative bacteria was orally administered in 5 female pigs and 8 male goats. Two of the gilts were pregnant. A solution of lipopolysaccharide (LPS) of Enterobacter agglomerans was mixed into the feed ration of the pigs (40 mg/animal), and given by gastric tube into the rumen of the goats (1-20 mg/animal). Jugular venous blood was collected and clinical signs, rectal temperature and WBC counts were recorded for 12-24 hours. Plasma concentrations of the major plasma metabolite of PGF2 alpha, 15-keto-13,14-dihydro-PGF2 alpha were determined in both species, progesterone concentration only in pigs. The pigs showed slight to severe signs of endotoxemia. Increases in rectal temperature and levels of the PGF2 alpha metabolite occurred in 3 gilts. Progesterone level and the total WBC counts remained unchanged. Differential counts followed irregular patterns mostly within the normal range. The goats showed slight signs of discomfort. Temperature increased in one animal. No other parameters were altered after the intake of LPS. The observations in pigs indicate that endotoxin either penetrated the intestinal barrier causing systemic endotoxemia or induced inflammatory reactions in the intestine activating inflammatory mediators.
将革兰氏阴性菌的内毒素经口给予5头雌性猪和8头雄性山羊。其中2头后备母猪已怀孕。将成团肠杆菌的脂多糖（LPS）溶液混入猪的饲料中（每头动物40毫克），并通过胃管给予山羊瘤胃（每头动物1 - 20毫克）。采集颈静脉血，并记录12 - 24小时的临床症状、直肠温度和白细胞计数。测定了两个物种中PGF2α的主要血浆代谢物15 - 酮 - 13,14 - 二氢 - PGF2α的血浆浓度，仅测定了猪的孕酮浓度。猪表现出轻度至重度的内毒素血症迹象。3头后备母猪的直肠温度和PGF2α代谢物水平升高。孕酮水平和白细胞总数保持不变。分类计数大多呈不规则模式，但大多在正常范围内。山羊表现出轻微的不适迹象。1只动物体温升高。摄入LPS后，没有其他参数发生改变。在猪身上的观察结果表明，内毒素要么穿透肠道屏障导致全身性内毒素血症，要么在肠道中诱导炎症反应，激活炎症介质。
