Talan M I, Engel B T
Laboratory of Behavioral Sciences, National Institute on Aging, Baltimore, Maryland 21224.
Am J Physiol. 1990 Jun;258(6 Pt 2):R1512-5. doi: 10.1152/ajpregu.1990.258.6.R1512.
Existing tethering systems intended for continuous recording of blood pressure incur two major problems: 1) vertical movements of the animal in the cage produce a significant error in measured pressure introduced by the change in distance between the pressure transducer and the highest point of the infusion system and 2) the necessity for repeated zero adjustment of the pressure transducer requires sedation of the animal and disconnection of the catheter. Continuous infusion and a one-way valve in the infusion line in proximity to the pressure transducer will eliminate the error of measurement related to vertical movement of the animal. A remotely controlled, two-way solenoid valve can close the catheter line and open the pressure transducer to atmospheric pressure, thereby permitting repeated zero adjustment without sedation of the animal and disconnection of the catheter. Eliminating the need to disconnect the catheter will significantly reduce the risk of adventitious infection.
1）动物在笼中的垂直运动会导致测量压力出现显著误差，这是由压力传感器与输液系统最高点之间的距离变化引起的；2）压力传感器需要反复进行零点校准，这就需要对动物进行镇静并断开导管。在靠近压力传感器的输液管路中采用连续输液和单向阀，将消除与动物垂直运动相关的测量误差。一个遥控的双向电磁阀可以关闭导管线路，并使压力传感器与大气相通，从而无需对动物进行镇静和断开导管就能反复进行零点校准。无需断开导管将显著降低意外感染的风险。
