Chen Jyh-Ping, Chiang Yun
Department of Chemical and Materials Engineering, Chang Gung University, Taoyuan 333, Taiwan, China.
J Nanosci Nanotechnol. 2010 Nov;10(11):7560-4. doi: 10.1166/jnn.2010.2829.
Nanofibrous membrane (NFM) intended as wound dressing was prepared by electrospinning polyurethane (PU) solution containing silver ion, followed by reduction of silver ion to silver nanoparticles. The electrospun PU membrane has high surface area-to-volume ratio, controlled evaporative water transmission rate, good fluid drainage ability, and excellent antimicrobial activity. With an aim to promote wound healing, collagen was grafted to fiber surface by low temperature oxygen plasma treatment, which could improve surface hydrophilicity and facilitate covalent binding of collagen molecules to the plasma-treated PU surface. A NFM with no bead formation was obtained with fiber diameters around 159 nm. The presence of embedded silver nanoparticles and surface-grafted collagen was confirmed qualitatively and quantitatively. After modification, the NFM's antimicrobial activity improved to approximately 100% inhibition of bacterial growth with concomitant increase of membrane water absorption ability, which facilitates its use as a functional wound dressing. From animal studies, the NFM was better than gauze and commercial collagen sponge wound dressing in wound healing rate.
通过静电纺丝含银离子的聚氨酯（PU）溶液，随后将银离子还原为银纳米颗粒，制备了用作伤口敷料的纳米纤维膜（NFM）。静电纺丝的PU膜具有高的表面积与体积比、可控的蒸发水传输速率、良好的液体引流能力和优异的抗菌活性。为了促进伤口愈合，通过低温氧等离子体处理将胶原蛋白接枝到纤维表面，这可以提高表面亲水性并促进胶原蛋白分子与经等离子体处理的PU表面的共价结合。获得了无珠形成的NFM，其纤维直径约为159nm。定性和定量地证实了嵌入的银纳米颗粒和表面接枝的胶原蛋白的存在。改性后，NFM的抗菌活性提高到约100%抑制细菌生长，同时膜的吸水能力增加，这有利于其用作功能性伤口敷料。从动物研究来看，NFM在伤口愈合速率方面优于纱布和商业胶原蛋白海绵伤口敷料。
