Key Laboratory of Plant Functional Genomics, Ministry of Education, Yangzhou University, Yangzhou, People's Republic of China.
Theor Appl Genet. 2011 Mar;122(5):915-23. doi: 10.1007/s00122-010-1498-z. Epub 2010 Dec 8.
The indica rice cultivar, Teqing, shows a high level of resistance to rice stripe virus (RSV). It is believed that this resistance is controlled by the gene, qSTV11(TQ). For positional cloning of the resistance gene, a set of chromosome single segment substitution lines (CSSSLs) was constructed, all of which had the genetic background of the susceptible japonica cultivar, Lemont, with different single substituted segments of Teqing on chromosome 11. By identifying the resistance of the CSSSLs-2006 in a field within a heavily diseased area, the resistance gene qSTV11(TQ) was mapped between the markers Indel7 and RM229. Furthermore, in that region, six new markers were developed and 52 subregion CSSSLs (CSSSLs-2007) were constructed. The natural infection experiment was conducted again at different sites, with two replicates used in each site in order to identify the resistance phenotypes of the CSSSLs-2007 and resistant/susceptible controls in 2007. Through the results of 2007, qSTV11(TQ) was localized in a region defined by the markers, CAPs1 and Indel4. In order to further confirm the position of qSTV11(TQ), another set of subregion CSSSLs (CSSSLs-2009) was constructed. Finally, qSTV11(TQ) was localized to a 55.7 kb region containing nine annotated genes according to the genome sequence of japonica Nipponbare. The relationship between qSTV11(TQ) and Stvb-i (Hayano-Saito et al. in Theor Appl Genet 101:59-63, 2000) and the reliability of the markers used on both sides of qSTV11(TQ) for marker-assisted breeding of resistance to rice stripe disease are discussed.
籼稻品种特青对水稻条纹病毒（RSV）表现出高水平的抗性。据信，这种抗性由基因 qSTV11（TQ）控制。为了对该抗性基因进行定位克隆，构建了一套染色体单片段代换系（CSSSLs），它们均具有感病粳稻品种 Lemont 的遗传背景，在第 11 号染色体上具有特青的不同单片段代换。通过在重疫区田间鉴定 CSSSLs-2006 的抗性，将 qSTV11（TQ）基因定位在标记 Indel7 和 RM229 之间。此外，在该区域开发了六个新标记，并构建了 52 个亚区 CSSSLs（CSSSLs-2007）。2007 年在不同地点再次进行自然感染实验，每个地点使用两个重复，以鉴定 CSSSLs-2007 的抗性表型和抗性/感病对照。通过 2007 年的结果，将 qSTV11（TQ）定位在由标记 CAPs1 和 Indel4 定义的区域内。为了进一步确认 qSTV11（TQ）的位置，构建了另一套亚区 CSSSLs（CSSSLs-2009）。最后，根据粳稻 Nipponbare 的基因组序列，将 qSTV11（TQ）定位到一个包含九个注释基因的 55.7 kb 区域内。讨论了 qSTV11（TQ）与 Stvb-i（Hayano-Saito 等人，Theor Appl Genet 101:59-63,2000）之间的关系，以及 qSTV11（TQ）两侧标记在水稻条纹病抗性标记辅助选择中的可靠性。
