Hasselaar P, Derksen R H, Blokzijl L, de Groot P G
Department of Haematology, University Hospital Utrecht, The Netherlands.
Thromb Haemost. 1990 Apr 12;63(2):169-73.
The interrelationship of antibodies directed against cardiolipin (CL), double stranded DNA (dsDNA), endothelial cells (EC) and blood platelets was investigated. IgG fractions, reactive with these antigens, were isolated from the plasmas from 8 patients with systemic lupus erythematosus and tested for crossreactivity with anionic phospholipids (CL, phosphatidylserine, phosphatidylinositol), zwitterionic phospholipids (phosphatidylethanolamine, phosphatidylcholine), dsDNA, EC and platelets by enzyme-linked immunosorbent assays and for lupus anticoagulant (LAC) activity with a coagulation assay. Our results demonstrate the frequent occurrence of crossreactivity between antibodies to anionic phospholipids, EC and platelets. Crossreactivities between these antibodies and antibodies to dsDNA or zwitterionic phospholipids are exceptional. LAC activity was found in the anti-CL, anti-EC and anti-platelet fractions of only one patient. These findings support the hypothesis that subpopulations of antibodies directed against negatively charged phospholipids can bind to EC and blood platelets, which may have implications for the pathogenetic potential of antiphospholipid antibodies.
研究了针对心磷脂（CL）、双链DNA（dsDNA）、内皮细胞（EC）和血小板的抗体之间的相互关系。从8例系统性红斑狼疮患者的血浆中分离出与这些抗原反应的IgG组分，并通过酶联免疫吸附试验检测其与阴离子磷脂（CL、磷脂酰丝氨酸、磷脂酰肌醇）、两性离子磷脂（磷脂酰乙醇胺、磷脂酰胆碱）、dsDNA、EC和血小板的交叉反应性，并用凝血试验检测狼疮抗凝物（LAC）活性。我们的结果表明，抗阴离子磷脂、EC和血小板的抗体之间经常出现交叉反应。这些抗体与抗dsDNA或两性离子磷脂抗体之间的交叉反应很少见。仅在1例患者的抗CL、抗EC和抗血小板组分中发现LAC活性。这些发现支持这样一种假说，即针对带负电荷磷脂的抗体亚群可与EC和血小板结合，这可能对抗磷脂抗体的致病潜力有影响。
