Immunology Division, Department of Health Sciences, Faculty of Experimental Sciences, University of Jaén, Jaén, Spain.
J Agric Food Chem. 2011 Jan 12;59(1):121-30. doi: 10.1021/jf102319y. Epub 2010 Dec 10.
This research aimed to investigate erythrodiol, uvaol, oleanolic acid, and maslinic acid scavenging capacities and their effects on cytotoxicity, cell proliferation, cell cycle, apoptosis, reactive oxygen species (ROS) level, and oxidative DNA damage on human MCF-7 breast cancer cell line. The results showed that erythrodiol, uvaol, and oleanolic acid have a significant cytotoxic effect and inhibit proliferation in a dose- and time-dependent manner. At 100 μM, erythrodiol growth inhibition occurred through apoptosis, with the observation of important ROS production and DNA damage, whereas uvaol and oleanolic acid growth inhibition involved cell cycle arrest. Moreover, although all tested triterpenes did not show free radical scavenging activity using ABTS and DPPH assays, they protected against oxidative DNA damage at the concentration 10 μM. Uvaol and oleanolic and maslinic acids, tested at 10 and 100 μM, also reduced intracellular ROS level and prevented H(2)O(2)-induced oxidative injury. Overall, the results suggest that tested triterpenes may have the potential to provide significant natural defense against human breast cancer.
本研究旨在探讨红景天二醇、羽扇豆醇、齐墩果酸和马桑酸的清除能力及其对人 MCF-7 乳腺癌细胞系细胞毒性、细胞增殖、细胞周期、细胞凋亡、活性氧 (ROS) 水平和氧化 DNA 损伤的影响。结果表明，红景天二醇、羽扇豆醇和齐墩果酸具有显著的细胞毒性作用，并呈剂量和时间依赖性抑制增殖。在 100 μM 时，红景天二醇通过细胞凋亡抑制生长，观察到重要的 ROS 产生和 DNA 损伤，而羽扇豆醇和齐墩果酸通过细胞周期阻滞抑制生长。此外，尽管所有测试的三萜类化合物在使用 ABTS 和 DPPH 测定法时均没有显示出自由基清除活性，但它们在 10 μM 的浓度下可防止氧化 DNA 损伤。在 10 和 100 μM 下测试的羽扇豆醇、齐墩果酸和马桑酸也降低了细胞内 ROS 水平，并防止了 H 2 O 2 诱导的氧化损伤。总体而言，研究结果表明，所测试的三萜类化合物可能具有为人体提供天然防御乳腺癌的潜力。
