Department of Chemistry, Nankai University, 94 Weijin Road, Tianjin 300071, China.
Biosens Bioelectron. 2011 Jan 15;26(5):2553-8. doi: 10.1016/j.bios.2010.11.004. Epub 2010 Nov 16.
A newly designed molecularly imprinted polymer (MIP) material was fabricated and successfully utilized as recognition element to develop a quantum dots (QDs) based MIP-coated composite for selective recognition of the template cytochrome c (Cyt). The composites were synthesized by sol-gel reaction (imprinting process). The imprinting process resulted in an increased affinity of the composites toward the corresponding template. The fluorescence of MIP-coated QDs was stronger quenched by the template versus that of non-imprinted polymer (NIP)-coated QDs, which indicated the composites could recognize the corresponding template. The results of specific experiments further exhibited the recognition ability of the composites. Under optimum conditions, the linear range for Cyt is from 0.97 μM to 24 μM, and the detection limit is 0.41 μM. The new composites integrated the high selectivity of molecular imprinting technology and fluorescence property of QDs and could convert the specific interactions between imprinted cavities and corresponding template to the obvious changes of fluorescence signal. Therefore, a simple and selective sensing system for protein recognition has been realized.
一种新型设计的分子印迹聚合物（MIP）材料被制备出来，并成功地用作识别元件，开发了一种基于量子点（QDs）的 MIP 涂层复合材料，用于选择性识别模板细胞色素 c（Cyt）。复合材料通过溶胶-凝胶反应（印迹过程）合成。印迹过程导致复合材料对相应模板的亲和力增加。与非印迹聚合物（NIP）涂层 QDs 相比，MIP 涂层 QDs 的荧光被模板更强地猝灭，这表明复合材料可以识别相应的模板。特定实验的结果进一步显示了复合材料的识别能力。在最佳条件下，Cyt 的线性范围为 0.97 μM 至 24 μM，检测限为 0.41 μM。新型复合材料集成了分子印迹技术的高选择性和 QDs 的荧光特性，可以将印迹腔与相应模板之间的特异性相互作用转化为荧光信号的明显变化。因此，实现了一种简单而选择性的蛋白质识别传感系统。
