Wang Yu, Wang Yuzhen, Liu Huilin
Department of Food Science and Engineering, Shanxi Agricultural University, Jinzhong 30600, China.
Department of Food and Chemical Engineering, Beijing Technology and Business University, Beijing 100048, China.
Nanomaterials (Basel). 2019 Feb 23;9(2):305. doi: 10.3390/nano9020305.
A fluorescence and solid phase extraction (SPE) adsorption nanomaterials of molecularly imprinted polymers (MIPs) based on quantum dot-grafted covalent organic frameworks (QD-grafted COFs) was prepared by one-pot surface-imprinting synthesis method. Amino groups of silane reagent were at the surface of QDs to coordinate COFs efficiently by Schiff-base reactions, providing thermal and chemical stability to MIPs. It also reacted with the phenolic hydroxyl groups of ferulic acid (FA) through non-covalent interactions. The nanomaterials were used as fluorescence sensing and SPE adsorption toward determination of ferulic acid. The MIPs based on QD-grafted COFs had good fluorescence response ability, and quenching linearly at concentrations of ferulic acid from 0.03 to 60 mg kg, with a detection limit of 5 µg kg. At the same time, it exhibited a good SPE adsorption ability, and the FA extraction was from 1.63 to 3.11 mg kg in grain by-products by SPE coupled with high performance liquid chromatography/mass spectrometry (HPLC/MS). The fluorescence and SPE-HPLC/MS were used for the efficient detection of ferulic acid in real samples with recovery values of 88⁻114% and 90⁻97%, respectively. Furthermore, the nanomaterials of MIPs based on QD-grafted COFs were used for FA detection with high sensitivity and selectivity, and it also increased the recycling of waste resources.
通过一锅法表面印迹合成法制备了一种基于量子点接枝共价有机框架（QD接枝COF）的分子印迹聚合物（MIP）荧光和固相萃取（SPE）吸附纳米材料。硅烷试剂的氨基位于量子点表面，通过席夫碱反应与COF有效配位，为MIP提供热稳定性和化学稳定性。它还通过非共价相互作用与阿魏酸（FA）的酚羟基反应。该纳米材料用作荧光传感和SPE吸附剂用于测定阿魏酸。基于QD接枝COF的MIP具有良好的荧光响应能力，在阿魏酸浓度为0.03至60 mg/kg时呈线性猝灭，检测限为5 μg/kg。同时，它表现出良好的SPE吸附能力，通过SPE结合高效液相色谱/质谱（HPLC/MS）从谷物副产品中提取FA的量为1.63至3.11 mg/kg。荧光和SPE-HPLC/MS用于实际样品中阿魏酸的高效检测，回收率分别为88%至114%和90%至97%。此外，基于QD接枝COF的MIP纳米材料用于FA检测具有高灵敏度和选择性，还提高了废弃物资源的再利用。
