Department of Biochemistry, Institute of Chemistry, Faculty of Science, P.J. Šafárik University, Moyzesova 11, 04167 Košice, Slovak Republic.
Int J Biol Macromol. 2011 Mar 1;48(2):319-25. doi: 10.1016/j.ijbiomac.2010.12.001. Epub 2010 Dec 8.
The interaction of a copper complexes containing Schiff bases with calf thymus (CT) DNA was investigated by spectroscopic methods. UV-vis, fluorescence and CD spectroscopies were conducted to assess their binding ability with CT DNA. The binding constants K have been estimated from 0.8 to 9.1×10(4) M(-1). The percentage of hypochromism is found to be over 70% (from spectral titrations). The results showed that the copper(II) complexes could bind to DNA with an intercalative mode. Synergic action of Cu(II) complexes with ascorbic acid against Candida albicans induced the generation of free radicals and increased (more than 60 times) antimicrobial effect of these complexes.
采用光谱法研究了含席夫碱的铜配合物与小牛胸腺 DNA 的相互作用。通过紫外可见光谱、荧光光谱和圆二色谱研究了它们与 CT DNA 的结合能力。从 0.8 到 9.1×10(4) M(-1) 估算了结合常数 K。从光谱滴定中发现，增色率超过 70%。结果表明，铜(II)配合物可以与 DNA 以插入模式结合。抗坏血酸与铜(II)配合物的协同作用对抗白色念珠菌，诱导自由基的产生，并提高这些配合物的抗菌作用（超过 60 倍）。
