Tice R R, Erexson G L, Shelby M D
Integrated Laboratory Systems, Research Triangle Park, NC 27709.
Mutat Res. 1990 Jun-Aug;234(3-4):187-93. doi: 10.1016/0165-1161(90)90014-f.
Studies are reviewed in which the effect of treatment/sample protocol on the induction of micronucleated polychromatic erythrocytes (MN-PCE) in male B6C3F1 mice by 3 carcinogens (benzidine, dimethylbenzanthracene and mitomycin C) were evaluated. 3 different treatment/sampling protocols were used, involving from 1 to 3 consecutive daily treatments and from 3 to 1, respectively, consecutive daily samplings beginning 24 h after the last injection. The results indicate that the 3-day injection/single sample time protocol eliminates the need for multiple sample times, minimizes the number of animals required in a study, decreases the time needed for data collection and simplifies data analysis. A comparison of the frequency of induced MN-PCE in peripheral blood and bone marrow suggests that, following a 3-injection protocol, either tissue can be used with equal efficiency.
对一些研究进行了综述，这些研究评估了处理/采样方案对3种致癌物（联苯胺、二甲基苯并蒽和丝裂霉素C）诱导雄性B6C3F1小鼠产生微核多染红细胞（MN-PCE）的影响。使用了3种不同的处理/采样方案，分别涉及连续1至3天的处理以及在最后一次注射后24小时开始连续3至1天的每日采样。结果表明，3天注射/单次采样时间方案消除了对多个采样时间的需求，将研究所需动物数量降至最低，减少了数据收集所需时间并简化了数据分析。外周血和骨髓中诱导产生的MN-PCE频率比较表明，按照3次注射方案，两种组织均可同等有效地使用。
