Goodwin M A, Lamichhane C M, Brown J, Smeltzer M A, Miller S L, Girshick T, Snyder D B, Dickson T G
Georgia Poultry Laboratory, Oakwood 30566.
Avian Dis. 1992 Jul-Sep;36(3):512-4.
Serum samples collected from breeder chickens ranging in age from 1 day to 55 weeks were tested for CAA antibodies by enzyme-linked immunosorbent assay (ELISA) and indirect immunofluorescent antibody (IFA) test. The relationship of ELISA to IFA test was determined. The sensitivity of the ELISA relative to the IFA test was 82.64%, and the specificity of the ELISA relative to the IFA test was 56.25%. Agreement between the ELISA and the IFA test was highly significant (Kappa = 0.74, Z = 5.78). We concluded that the ELISA is as good as the IFA test for detecting CAA antibody in sera from chickens.
