Lamichhane C M, Snyder D B, Girschick T, Goodwin M A, Miller S L
Virginia-Maryland Regional College of Veterinary Medicine, University of Maryland, College Park 20742.
Avian Dis. 1992 Jul-Sep;36(3):725-9.
This paper describes the development of an indirect immunoperoxidase assay (IIP) and an indirect enzyme-linked immunosorbent assay (ELISA) for detecting antibodies to chicken anemia virus (VAC). The IIP assay developed used CAV-infected MDCC-MSB1 cells for detecting antibody to CAV, whereas the ELISA utilized gradient-purified immunoadsorbed CAV as the target antigen. The IIP and ELISA were compared with the standard indirect immunofluorescent antibody (IFA) assay, which is more conventionally used to screen chicken serum for antibodies against CAV. Comparative test results of 185 field samples of chicken serum by these three methods were in agreement 84% of the time. Both IFA and IIP assays yielded fewer positive tests than did the ELISA. IFA and IIP assays were in agreement 93% of the time, as compared with 91% agreement of IIP and ELISA results, or 84% agreement for comparative IFA and ELISA results.
本文描述了一种用于检测鸡贫血病毒（CAV）抗体的间接免疫过氧化物酶试验（IIP）和间接酶联免疫吸附试验（ELISA）的开发。所开发的IIP试验使用感染CAV的MDCC-MSB1细胞来检测CAV抗体，而ELISA则利用梯度纯化的免疫吸附CAV作为靶抗原。将IIP和ELISA与标准间接免疫荧光抗体（IFA）试验进行比较，后者更常用于筛查鸡血清中抗CAV的抗体。这三种方法对185份鸡血清现场样本的比较测试结果在84%的时间内是一致的。IFA和IIP试验产生的阳性测试结果均少于ELISA。IFA和IIP试验在93%的时间内结果一致，相比之下，IIP和ELISA结果的一致性为91%，IFA和ELISA比较结果的一致性为84%。
