Embellisia 和 Nimbya 物种的分子特征及其与 Alternaria、Ulocladium 和 Stemphylium 的关系。

Molecular characterization of Embellisia and Nimbya species and their relationship to Alternaria, Ulocladium and Stemphylium.

机构信息

Department of Plant Pathology, Forbes 204, University of Arizona, Tucson, Arizona 85721-0036.

出版信息

Mycologia. 2003 Nov-Dec;95(6):1141-54. doi: 10.1080/15572536.2004.11833024.

DOI:10.1080/15572536.2004.11833024

Abstract

DNA sequences from rDNA and protein-coding regions were determined for six Embellisia and two Nimbya spp. and were compared to those from Alternaria, Ulocladium and Stemphylium spp. Sequences determined included rDNA from the nuclear internal transcribed-spacer region (ITS1/5.8S/ITS2) and the mitochondrial small-subunit (mt SSU) and a portion of the glyceraldehyde-3-phosphate dehydrogenase (gpd) gene. Phylogenetic analyses were performed on each dataset separately and then combined for total evidence analysis using methods of maximum parsimony and maximum likelihood. Results revealed that Embellisia and Nimbya clustered within a large monophyletic Alternaria-Nimbya-Embellisia-Ulocladium clade with Stemphylium as the sister taxon. Members of the infectoria species-group were the most basal group in this large polygeneric clade. Embellisia and Nimbya were sister taxa of the remaining Alternaria and Ulocladium spp. and were related more closely to Alternaria than was Stemphylium. Four Embellisia spp. formed a monophyletic clade. However, E. allii clustered with the two Nimbya spp. and E. indefessa clustered with Alternaria and Ulocladium spp., revealing that Embellisia, as currently circumscribed, is polyphyletic. Potential revisions of taxonomy for all genera are discussed.

摘要

从 rDNA 和蛋白质编码区确定了六个 Embellisia 和两个 Nimbya 种的 DNA 序列，并与 Alternaria、Ulocladium 和 Stemphylium 种的序列进行了比较。确定的序列包括核内转录间隔区（ITS1/5.8S/ITS2）和线粒体小亚基（mt SSU）的 rDNA 以及甘油醛-3-磷酸脱氢酶（gpd）基因的一部分。分别对每个数据集进行了系统发育分析，然后使用最大简约法和最大似然法对总证据进行了联合分析。结果表明，Embellisia 和 Nimbya 聚集成一个大的单系 Alternaria-Nimbya-Embellisia-Ulocladium 分支，Stemphylium 是姐妹分类群。infectoria 种组的成员是这个大的多属分支中最基础的组。Embellisia 和 Nimbya 是其余的 Alternaria 和 Ulocladium 种的姐妹分类群，与 Stemphylium 相比，与 Alternaria 的关系更密切。四个 Embellisia 种形成了一个单系分支。然而，Allii 与两个 Nimbya 种聚类，而 indefessa 与 Alternaria 和 Ulocladium 种聚类，表明 Embellisia 目前的定义是多系的。讨论了所有属的分类修订的潜力。