Gannibal Philipp B, Orina Aleksandra S, Kononenko Galina P, Burkin Aleksey A
Laboratory of Mycology and Phytopathology, All-Russian Institute of Plant Protection, 196608 St. Petersburg, Russia.
Mycotoxicology Laboratory, All-Russia Research Institute of Veterinary Sanitation, Hygiene, and Ecology-Skryabin and Kovalenko Federal Scientific Center, All-Russia Research Institute of Experimental Veterinary Medicine, 123022 Moscow, Russia.
J Fungi (Basel). 2022 Apr 20;8(5):423. doi: 10.3390/jof8050423.
Species of the genus are ubiquitous and frequently isolated from various plants, including crops. There are two phylogenetically and morphologically close sections: the relatively well-known and the rarely mentioned . Currently, the latter includes at least seven species that are less studied and sometimes misidentified. To perform precise identification, two primers (APsF and APsR) were designed and a sect. -specific PCR method was developed. Thirty-five Russian . -like strains were then examined. Five strains were found to be the members of the sect. . Additionally, specificity of the previously developed primer set (Ain3F and Ain4R) was checked. It was found to be highly specific for sect. and did not amplify sect. DNA. Identification of strains of the sect. was supported and refined by phylogenetic reconstruction based on analysis of two loci, the glyceraldehyde-3-phosphate dehydrogenase gene (), and the plasma membrane ATPase gene (). These fungi belonged to and , which were the first detection of those taxa for the Eastern Europe. was isolated from cereal seeds and eleuthero leaves. was obtained from oat seed. All strains of sect. were not able to produce alternariol mycotoxin, as well as the majority of sect. strains.
该属的物种广泛存在，经常从包括农作物在内的各种植物中分离得到。有两个在系统发育和形态上相近的组：相对知名的组和很少被提及的组。目前，后者包括至少七个研究较少且有时被误认的物种。为了进行精确鉴定，设计了两种引物（APsF和APsR）并开发了一种组特异性PCR方法。然后对35株俄罗斯类菌株进行了检测。发现有五株是组的成员。此外，还检查了先前开发的引物组（Ain3F和Ain4R）的特异性。发现它对组具有高度特异性，不会扩增组的DNA。基于对甘油醛-3-磷酸脱氢酶基因（）和质膜ATPase基因（）两个位点的分析，通过系统发育重建支持并完善了组菌株的鉴定。这些真菌属于和，这是在东欧首次检测到这些分类群。从谷物种子和刺五加叶中分离得到。从燕麦种子中获得。组的所有菌株以及组的大多数菌株都不能产生交链孢酚霉菌毒素。
