Institut National de Santé et de Recherche Médicale, Unité 625, Institut Fédératif de Recherche 140, Université de Rennes 1, F-35042 Rennes, France.
Proc Natl Acad Sci U S A. 2011 Jan 18;108(3):1058-63. doi: 10.1073/pnas.1016459108. Epub 2010 Dec 13.
Budding yeast noncoding RNAs (ncRNAs) are pervasively transcribed during mitosis, and some regulate mitotic protein-coding genes. However, little is known about ncRNA expression during meiotic development. Using high-resolution profiling we identified an extensive meiotic ncRNA expression program interlaced with the protein-coding transcriptome via sense/antisense transcript pairs, bidirectional promoters, and ncRNAs that overlap the regulatory regions of genes. Meiotic unannotated transcripts (MUTs) are mitotic targets of the conserved exosome component Rrp6, which itself is degraded after the onset of meiosis when MUTs and other ncRNAs accumulate in successive waves. Diploid cells lacking Rrp6 fail to initiate premeiotic DNA replication normally and cannot undergo efficient meiotic development. The present study demonstrates a unique function for budding yeast Rrp6 in degrading distinct classes of meiotically induced ncRNAs during vegetative growth and the onset of meiosis and thus points to a critical role of differential ncRNA expression in the execution of a conserved developmental program.
芽殖酵母非编码 RNA（ncRNA）在有丝分裂期间广泛转录，其中一些调节有丝分裂蛋白编码基因。然而，关于减数分裂发育过程中 ncRNA 表达的知之甚少。使用高分辨率分析，我们通过 sense/antisense 转录对、双向启动子以及与基因调控区域重叠的 ncRNA，鉴定出广泛的减数分裂 ncRNA 表达程序与蛋白质编码转录组交织在一起。减数分裂未注释转录物（MUT）是保守外切体成分 Rrp6 的有丝分裂靶标，当 MUT 和其他 ncRNA 以连续波的形式积累时，Rrp6 自身在减数分裂开始时降解。缺乏 Rrp6 的二倍体细胞不能正常启动前期 DNA 复制，也不能进行有效的减数分裂发育。本研究证明了芽殖酵母 Rrp6 在降解不同类别的有丝分裂诱导 ncRNA 方面的独特功能，这些 ncRNA 在营养生长和减数分裂开始时积累，因此表明差异 ncRNA 表达在执行保守的发育程序中起着关键作用。
