一种与Oct4假基因5反义的长链非编码RNA对Oct4的转录调控。
Transcriptional regulation of Oct4 by a long non-coding RNA antisense to Oct4-pseudogene 5.
作者信息
Hawkins Peter G, Morris Kevin V
机构信息
Department of Molecular and Experimental Medicine; The Scripps Research Institute; La Jolla, CA USA.
出版信息
Transcription. 2010 Nov;1(3):165-175. doi: 10.4161/trns.1.3.13332. Epub 2010 Aug 16.
Abstract
Long non-coding RNAs (lncRNAs) have been shown to epigenetically regulate certain genes in human cells. Here we report evidence for the involvement of an antisense lncRNA in the transcriptional regulation of the pluripotency-associated factor Oct4. When an lncRNA antisense to Oct4-pseudogene 5 was suppressed, transcription of Oct4 and Oct4 pseudogenes 4 and 5 was observed to increase. This increase correlated with a loss of silent state epigenetic marks and the histone methyltransferase Ezh2 at the Oct4 promoter. We observed this lncRNA to interact with nucleolin and PURA, a 35 kD single-stranded DNA and RNA binding protein, and found that these proteins may act to negatively regulate this antisense transcript.
摘要
长链非编码RNA（lncRNAs）已被证明可在人类细胞中对某些基因进行表观遗传调控。在此，我们报告了一种反义lncRNA参与多能性相关因子Oct4转录调控的证据。当抑制与Oct4假基因5反义的lncRNA时，观察到Oct4以及Oct4假基因4和5的转录增加。这种增加与Oct4启动子处沉默状态表观遗传标记和组蛋白甲基转移酶Ezh2的缺失相关。我们观察到这种lncRNA与核仁素以及PURA（一种35kD的单链DNA和RNA结合蛋白）相互作用，并发现这些蛋白可能对这种反义转录本起负调控作用。
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