School of Health Sciences, University of Wollongong, NSW 2522, Australia; E-Mail:
Int J Mol Sci. 2010 Oct 15;11(10):3954-64. doi: 10.3390/ijms11103954.
The aim of this study was to examine the effect of endurance training on skeletal muscle phospholipid molecular species from high-fat fed rats. Twelve female Sprague-Dawley rats were fed a high-fat diet (78.1% energy). The rats were randomly divided into two groups, a sedentary control group and a trained group (125 min of treadmill running at 8 m/min, 4 days/wk for 4 weeks). Forty-eight hours after their last training bout phospholipids were extracted from the red and white vastus lateralis and analyzed by electrospray-ionization mass spectrometry. Exercise training was associated with significant alterations in the relative abundance of a number of phospholipid molecular species. These changes were more prominent in red vastus lateralis than white vastus lateralis. The largest observed change was an increase of ~30% in the abundance of 1-palmitoyl-2-linoleoyl phosphatidylcholine ions in oxidative fibers. Reductions in the relative abundance of a number of phospholipids containing long-chain n-3 polyunsaturated fatty acids were also observed. These data suggest a possible reduction in phospholipid remodeling in the trained animals. This results in a decrease in the phospholipid n-3 to n-6 ratio that may in turn influence endurance capacity.
本研究旨在探讨耐力训练对高脂肪喂养大鼠骨骼肌磷脂分子种类的影响。12 只雌性 Sprague-Dawley 大鼠喂食高脂肪饮食（78.1%能量）。大鼠随机分为两组，即安静对照组和训练组（125 分钟 8m/min 的跑步机跑步，每周 4 天，共 4 周）。最后一次训练后 48 小时，从红、白股外侧肌提取磷脂，并通过电喷雾电离质谱进行分析。运动训练与多种磷脂分子种类的相对丰度的显著变化有关。这些变化在红股外侧肌比白股外侧肌更为明显。观察到的最大变化是氧化纤维中 1-棕榈酰-2-亚油酸磷脂酰胆碱离子的丰度增加了约 30%。还观察到含有长链 n-3 多不饱和脂肪酸的许多磷脂的相对丰度降低。这些数据表明，训练动物的磷脂重塑可能减少。这导致磷脂 n-3 与 n-6 比值降低，这可能反过来影响耐力。
