Kraitchman Dara L, Kedziorek Dorota A, Bulte Jeff W M
Division of MR Research, Russell H. Morgan Department of Radiology and Radiological Science, The Johns Hopkins University School of Medicine, Baltimore, MD, USA.
Methods Mol Biol. 2011;680:141-52. doi: 10.1007/978-1-60761-901-7_10.
Recently, several protocols for labeling of stem cells with superparamagnetic iron oxides (SPIOs) have been developed, leading to an active and growing field aimed at visualizing stem cells using MRI (magnetic resonance imaging), including image-guided stem cell injections. This development occurred simultaneously with a significant rise in the number of cell therapy clinical trials for cardiovascular applications and their preceding pre-clinical studies in animal models. In this chapter, we will describe several labeling strategies that can be used to label cells with SPIO nanoparticles. This is followed by a discussion of current strategies for using MRI to visualize these cells in myocardial infarct.
最近，已经开发出几种用超顺磁性氧化铁（SPIO）标记干细胞的方案，这导致了一个活跃且不断发展的领域，旨在利用磁共振成像（MRI）对干细胞进行可视化，包括图像引导的干细胞注射。这一发展与心血管应用细胞治疗临床试验数量及其之前在动物模型中的临床前研究数量的显著增加同时发生。在本章中，我们将描述几种可用于用SPIO纳米颗粒标记细胞的标记策略。接下来将讨论目前在心肌梗死中利用MRI对这些细胞进行可视化的策略。
