Ludwig Boltzmann Institute for Experimental and Clinical Traumatology, Austrian Cluster for Tissue Regeneration, Vienna, Austria.
Surg Endosc. 2011 Jul;25(7):2125-31. doi: 10.1007/s00464-010-1507-y. Epub 2010 Dec 10.
Various antiadhesive coatings have been proposed for intraperitoneal onlay meshes (IPOM). However, adhesions, mesh infections, and impaired integration remain clinically relevant problems. In this experiment, human vital amniotic membrane (AM) was tested as antiadhesive mesh coating. Vital AM complies with clinical standards of product safety.
In this study, 24 rats were randomized to one control or two treatment groups (n=8). An uncoated polypropylene mesh (Vitamesh) was implanted using open IPOM technique and fixed with four sutures. In the treatment groups, vital AM was attached to Vitamesh by fibrin sealant fixation. The observation period was 7 and 17 days. Vitamesh fixed by suture only served as the control condition (17 days). Adhesion formation, tissue integration, and neovascularization were assessed macroscopically and histologically.
All the meshes in the control group elicited severe adhesions. Vital AM was highly efficient in reducing adhesions to mesh and sutures. No foreign body reaction or unfavorable immunologic response to vital AM occurred. Tissue integration and neovascularization of coated meshes were good. Fibrin sealant yielded a reliable fixation.
Human vital AM was highly effective in reducing adhesions to polypropylene mesh and sutures in experimental IPOM. No adverse effects were detected, and tissue integration of the mesh was good.
已经提出了各种用于腹腔内补片（IPOM）的防粘连涂层。然而，粘连、网感染和受损的整合仍然是临床上相关的问题。在这项实验中，人类有生命力的羊膜（AM）被测试为防粘连网涂层。有生命力的 AM 符合产品安全的临床标准。
在这项研究中，24 只大鼠随机分为对照组或两个治疗组（n=8）。使用开放式 IPOM 技术植入未涂层的聚丙烯网（Vitamesh），并用四个缝线固定。在治疗组中，通过纤维蛋白胶固定将有生命力的 AM 附着在 Vitamesh 上。观察期为 7 天和 17 天。仅用缝线固定的 Vitamesh 作为对照条件（17 天）。通过宏观和组织学评估粘连形成、组织整合和新生血管形成。
对照组的所有网都引起了严重的粘连。有生命力的 AM 非常有效地减少了网和缝线的粘连。没有发生异物反应或对有生命力的 AM 的不利免疫反应。涂层网的组织整合和新生血管形成良好。纤维蛋白胶产生了可靠的固定。
人类有生命力的 AM 非常有效地减少了实验性 IPOM 中聚丙烯网和缝线的粘连。未发现不良反应，网的组织整合良好。
