Department of Pharmaceutical Sciences, University of Florence, Via Ugo Schiff 6, 50019 Sesto Fiorentino, Firenze, Italy.
Anal Bioanal Chem. 2011 Feb;399(4):1715-23. doi: 10.1007/s00216-010-4473-2. Epub 2010 Dec 12.
In the present study, a method based on liquid chromatography with diode array detection (HPLC-DAD) coupled to an electrospray ionisation (ESI) interface was developed for the determination of the constituents in the aqueous preparations of Viola odorata L. flowering tops. The developed assay was fast, simple and effective and permitted the quality control of the preparations. The aim of this work was to assess the qualitative and quantitative profile of the investigated preparations, which find until today wide applications in food and cosmetic industry, and to propose a validated method for their quality control. Characteristic constituents of V. odorata flowers are considered to be the anthocyanins; however, a detailed literature research showed that data concerning their chemical content are scarce. HPLC-DAD-ESI-MS analyses supported by extensive preparative chromatographic investigations and 2D NMR analyses revealed the predominance of complex flavonol glycosides and permitted the complete characterisation of the content of V. odorata preparations. This is the first report of detailed analysis of the chemical composition of V. odorata flowers.
在本研究中，建立了一种基于二极管阵列检测（HPLC-DAD）与电喷雾离子化（ESI）接口联用的液相色谱法，用于测定堇菜属植物开花顶端的水提制剂中的成分。所建立的方法快速、简单、有效，可用于制剂的质量控制。本工作的目的是评估所研究制剂的定性和定量特征，这些制剂至今在食品和化妆品行业得到广泛应用，并提出一种经过验证的质量控制方法。堇菜属植物花朵的特征成分被认为是花色苷；然而，详细的文献研究表明，关于其化学含量的数据很少。HPLC-DAD-ESI-MS 分析结合广泛的制备色谱研究和 2D NMR 分析表明，复杂的黄酮醇糖苷占优势，并允许对堇菜属植物制剂的含量进行完全表征。这是首次详细分析堇菜属植物花的化学成分的报告。
