Aslani Abolfazl, Asghari Golamreza, Darani Hossein Yosofi, Ghanadian Mustafa, Hosseini Fateme
Department of Pharmaceutics, School of Pharmacy and Pharmaceutical Sciences, Novel Drug Delivery Systems Research Center, Isfahan University of Medical Sciences, Isfahan, Iran.
Department of Pharmacognosy, School of Pharmacy and Pharmaceutical Sciences, Pharmaceutical Sciences Research Center, Isfahan University of Medical Sciences, Isfahan, Iran.
Int J Prev Med. 2019 Oct 9;10:179. doi: 10.4103/ijpvm.IJPVM_525_17. eCollection 2019.
Trichomoniasis is a common sexually transmitted disease that is caused by infection with protozoan parasite called . Metronidazole is the drug of choice for the treatment of this infection. In this study, design, formulation, and physicochemical evaluation of vaginal cream containing , and extracts for the prevention and treatment of trichomoniasis has been investigated.
Ethyl acetate extract of leaves, water fraction of root, and hydroalcoholic extract of leaves was prepared and used for anti-trichomonas experiments. Then, based on results, different formulations of vaginal cream containing mixed extracts were prepared and physicochemical evaluation was conducted. In the next step, anti-trichomonas effect of selective formulation was tested .
The mixed concentrates containing 2.5 mg/ml , 0.06 mg/ml , and 1 mg/ml showed 100% growth inhibition (GI) during 24 h. Furthermore, the mixture containing 1.25 mg/ml , 0.03 mg/ml , and 0.5 mg/ml showed 92% GI in the first 24 h. The selective formulation passed all of physicochemical test and also showed 100% GI for anti-trichomonas experiments in the first 24 h.
The mixed concentrates containing 2.5 mg/ml , 0.06 mg/ml of , and 1 mg/ml of are the mixture which showed the highest percentage of GI (100%) after 24 h. The selective formulation of vaginal cream containing this mixture of extracts was detected 100% GI in the first 24 h.
滴虫病是一种常见的性传播疾病，由一种名为[滴虫名称未给出]的原生动物寄生虫感染引起。甲硝唑是治疗这种感染的首选药物。在本研究中，对含有[植物名称未给出]叶、[植物名称未给出]根和[植物名称未给出]叶提取物用于预防和治疗滴虫病的阴道乳膏进行了设计、配方和理化性质评估。
制备了[植物名称未给出]叶的乙酸乙酯提取物、[植物名称未给出]根的水相部分和[植物名称未给出]叶的水醇提取物，并用于抗滴虫实验。然后，根据结果制备了含有混合提取物的不同配方的阴道乳膏，并进行了理化性质评估。下一步，测试了选择性配方的抗滴虫效果。
含有2.5mg/ml[成分名称未给出]、0.06mg/ml[成分名称未给出]和1mg/ml[成分名称未给出]的混合浓缩物在24小时内显示出100%的生长抑制（GI）。此外，含有1.25mg/ml[成分名称未给出]、0.03mg/ml[成分名称未给出]和0.5mg/ml[成分名称未给出]的混合物在前24小时内显示出92%的GI。选择性配方通过了所有理化测试，并且在抗滴虫实验的前24小时内也显示出100%的GI。
含有2.5mg/ml[成分名称未给出]、0.06mg/ml[成分名称未给出]和1mg/ml[成分名称未给出]的混合浓缩物是在24小时后显示出最高GI百分比（100%） 的混合物。含有这种提取物混合物的阴道乳膏的选择性配方在前24小时内检测到100%的GI。
