Protein separation on a polar-copolymerized C8 stationary phase.

A novel polar-copolymerized C(8) stationary phase composed of octyl and chloropropyl (C(8)-C(3)Cl)-bonded silica has been developed for protein separation. Separation of standard proteins on this homemade column was investigated and compared with separation on a Waters Symmetry 300 C(4) column. Results indicated that the chromatographic performance of the homemade column in the separation of proteins was excellent. The new polar copolymerized C(8) stationary phase enabled glycoprotein separation with good resolution and reproducibility. It was also used for purification of ovalbumin glycoprotein in order to improve identification of the protein.