Ghiran Ionita C
Department of Medicine, Beth Israel Deaconess Medical Center, Harvard Medical School, Boston, MA, USA.
Methods Mol Biol. 2011;689:93-136. doi: 10.1007/978-1-60761-950-5_7.
This chapter is an overview of basic principles of fluorescence microscopy, including a brief history on the invention of this type of microscopy. The chapter highlights important points related to properties of fluorochromes, resolution in fluorescence microscopy, phase contrast and fluorescence, fluorescence filters, construction of a fluorescence microscope, and tips on the correct use of this equipment.
本章概述了荧光显微镜的基本原理，包括此类显微镜发明的简要历史。本章重点介绍了与荧光染料特性、荧光显微镜分辨率、相差与荧光、荧光滤光片、荧光显微镜构造以及该设备正确使用技巧等相关的要点。
