使用不同的光学显微镜技术对白细胞内的脂质体进行成像。
Imaging lipid bodies within leukocytes with different light microscopy techniques.
作者信息
Melo Rossana C N, D'Ávila Heloisa, Bozza Patricia T, Weller Peter F
机构信息
Laboratory of Cellular Biology, Department of Biology, Federal University of Juiz de Fora, Juiz de Fora, MG, Brazil.
出版信息
Methods Mol Biol. 2011;689:149-61. doi: 10.1007/978-1-60761-950-5_9.
Abstract
Lipid bodies, also known as lipid droplets, are present in most eukaryotic cells. In leukocytes, lipid bodies are functionally active organelles with central roles in inflammation and are considered structural markers of inflammatory cells in a range of diseases. The identification of lipid bodies has methodological limitations because lipid bodies dissipate upon drying or dissolve upon fixation and staining with alcohol-based reagents. Here we discuss several techniques to detect and visualize lipid bodies within leukocytes by light microscopy. These techniques include staining with osmium or use of different fluorescent probes such as Nile red, BODIPY, Oil red, P96 and immunofluorescence labeling for adipose differentiation-related protein (ADRP).
摘要
脂体，也称为脂质滴，存在于大多数真核细胞中。在白细胞中，脂体是具有功能活性的细胞器，在炎症中起核心作用，并且在一系列疾病中被视为炎症细胞的结构标志物。脂体的鉴定存在方法学上的局限性，因为脂体会在干燥时消散，或在用基于酒精的试剂固定和染色时溶解。在这里，我们讨论几种通过光学显微镜检测和观察白细胞内脂体的技术。这些技术包括锇染色或使用不同的荧光探针，如尼罗红、硼二吡咯、油红、P96，以及对脂肪分化相关蛋白（ADRP）进行免疫荧光标记。
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