Xia Yong-gang, Liang Jun, Yang Bing-you, Wang Qiu-hong, Kuang Hai-xue
Key Laboratory of Chinese Materia Medica (Heilongjiang University of Chinese Medicine), Ministry of Education, Harbin, 150040, China.
Biomed Chromatogr. 2011 Sep;25(9):1030-7. doi: 10.1002/bmc.1564. Epub 2010 Dec 10.
A new method using capillary zone electrophoresis was developed for the rapid quantification of two common uronic acids, galacturonic acid and glucuronic acid, based on utilization of an alkaline background electrolyte with reversed electroosmotic flow (EOF) within 16 min. The method relies on in-capillary reaction and direct UV detection at the wavelength 270 nm. The optimum electrolyte solution was prepared of 130 mm sodium hydroxide, 36 mm disodium hydrogen phosphate dihydrate and 0.5 mm cetyltrimethylammonium bromide. EOF was reversed to detect uronic acids and to improve the separation of neutral sugars. The established method was validated and the results showed good linearity, high precision and satisfactory sensitivity. The newly developed method was successfully applied to analyze galacturonic acid and glucuronic acid content in Forsythia suspensa polysaccharides. The method is fast since only sample hydrolysis and dilution are required in the sample preparation.
基于在16分钟内利用具有反向电渗流（EOF）的碱性背景电解质，开发了一种使用毛细管区带电泳的新方法，用于快速定量两种常见的糖醛酸，即半乳糖醛酸和葡萄糖醛酸。该方法依赖于毛细管内反应和在270nm波长处的直接紫外检测。最佳电解质溶液由130mM氢氧化钠、36mM二水合磷酸氢二钠和0.5mM十六烷基三甲基溴化铵配制而成。通过反转EOF来检测糖醛酸并改善中性糖的分离。对所建立的方法进行了验证，结果显示出良好的线性、高精度和令人满意的灵敏度。新开发的方法成功应用于分析连翘多糖中的半乳糖醛酸和葡萄糖醛酸含量。该方法速度快，因为样品制备仅需要样品水解和稀释。
