重链类别转换不会终止体细胞突变。
Heavy-chain class switch does not terminate somatic mutation.
作者信息
Shan H, Shlomchik M, Weigert M
机构信息
Institute for Cancer Research, Fox Chase Cancer Center, Philadelphia, Pennsylvania 19111.
出版信息
J Exp Med. 1990 Aug 1;172(2):531-6. doi: 10.1084/jem.172.2.531.
Abstract
We studied the H-chain class switch rearrangement in four groups of clonally related B cell hybridomas, to test the hypothesis that class switch terminates somatic mutation in a B cell. Using switch region-specific probes in Southern blot analysis individual mu-gamma switch rearrangement events can be distinguished. We show that clonally related IgG-producing hybridomas that differ by mutations often share a common switch rearrangement. This indicates that class switch in these cells did not terminate somatic mutation.
摘要
我们研究了四组克隆相关的B细胞杂交瘤中的重链类别转换重排，以检验类别转换终止B细胞体细胞突变这一假说。在Southern印迹分析中使用特定于转换区的探针，可以区分单个μ-γ转换重排事件。我们发现，因突变而不同的克隆相关的产生IgG的杂交瘤常常共享一个共同的转换重排。这表明这些细胞中的类别转换并未终止体细胞突变。
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