Department of Plant Pathology, University of Stellenbosch, P. Bag X1, Matieland 7602, South Africa.
Mycologia. 2003 Mar-Apr;95(2):294-307.
Botryosphaeria spp. occur on and cause diseases of Proteaceae, but accurate identification has been problematic due to the lack of clear species circumscriptions of members of this genus. In this study, 46 isolates of Botryosphaeria from proteaceous hosts growing in various parts of the world were studied, using morphology, cultural characters and sequence data from the ITS region of the rDNA operon. Five Botryosphaeria spp. were found to be associated with Proteaceae. Botryosphaeria lutea was isolated from Banksia and Buckinghamia spp. in Australia, and a single isolate was obtained from Protea cynaroides in South Africa. Botryosphaeria proteae was associated only with South African Proteaceae, but occurred in many parts of the world. Another Botryosphaeria sp. that occurred exclusively on South African Proteaceae represents a new taxon that is described as B. protearum. This pathogen was found on South African Proteaceae cultivated in Australia; Hawaii; Portugal, including the Madeira Islands; and South Africa. Botryosphaeria ribis was associated with both South African and Australian Proteaceae and was isolated from material collected in Australia, Hawaii and Zimbabwe. A single occurrence of B. obtusa as an endophyte was recorded from P. magnifica in South Africa. In addition to providing a taxonomic overview of Botryosphaeria spp. associated with Proteaceae, this paper clarifies for the first time the global distribution of these species. A key also is provided to facilitate their identification. A large number of new host and distribution records are made and a new species of Botryosphaeria from Proteaceae is described.
球腔菌属 Botryosphaeria 物种存在于并引起保护科植物的疾病，但由于该属成员缺乏明确的物种界限，因此准确鉴定一直存在问题。在这项研究中，使用形态学、培养特征和 rDNA 操纵子 ITS 区的序列数据，研究了来自世界各地不同部位的 46 个保护科植物宿主的 Botryosphaeria 分离物。发现有 5 种 Botryosphaeria 物种与保护科植物有关。B. lutea 从澳大利亚的 Banksia 和 Buckinghamia 属中分离出来，在南非从 Protea cynaroides 中分离出一个单一的分离物。Botryosphaeria proteae 仅与南非保护科植物有关，但在世界许多地方都有发生。另一种仅发生在南非保护科植物上的 Botryosphaeria sp. 是一个新的分类群，被描述为 B. protearum。该病原体出现在澳大利亚、夏威夷、葡萄牙（包括马德拉群岛）和南非种植的南非保护科植物上。Botryosphaeria ribis 与南非和澳大利亚的保护科植物有关，并从澳大利亚、夏威夷和津巴布韦收集的材料中分离出来。在南非的 P. magnifica 中记录到了 B. obtusa 作为内生菌的单一发生。除了提供与保护科植物有关的 Botryosphaeria spp. 的分类概述外，本文还首次阐明了这些物种的全球分布。还提供了一个关键，以方便它们的鉴定。本文还记录了大量新的宿主和分布记录，并描述了来自保护科植物的一个新的 Botryosphaeria 物种。
