Department of Microbiology & Plant Pathology, Forestry & Agricultural Biotechnology Institute (FABI), University of Pretoria, Pretoria, 0001 South Africa.
Mycologia. 2003 May-Jun;95(3):434-41.
Ophiostoma stenoceras is a well-known sapwood-colonizing fungus occurring on some coniferous and hardwood hosts in the Northern Hemisphere. In the Southern Hemisphere, the fungus has been reported only from New Zealand. The human pathogen, Sporothrix schenckii, has been suggested to be the anamorph of O. stenoceras. The aim of this study was to gain a better understanding of the phylogenetic relationship between these two species. The study also provided the opportunity to confirm the identity of some Sporothrix and O. stenoceras-like isolates recently collected from wood and soil around the world. For this purpose, the DNA sequence of internal transcribed spacer (ITS) regions of the ribosomal RNA operon was determined. Isolates of O. nigrocarpum, O. albidum, O. abietinum, O. narcissi and O. ponderosae, all morphologically similar to O. stenoceras, were included in the study. From phylogenetic analyses of the sequence data, four main clades were observed. These represented O. stenoceras, O. nigrocarpum and two separate groups containing isolates of S. schenckii. Our results confirm earlier suggestions that S. schenckii should be classified within the teleomorph genus Ophiostoma but support studies separating O. stenoceras and S. schenckii. Ophiostoma albidum and O. ponderosae should be considered synonyms of O. stenoceras. The status of O. narcissi and O. abietinum needs further clarification. The two groups within S. schenckii might represent two species, but this needs to be confirmed. This study represents the first reports of O. stenoceras from Colombia, Kenya, Uruguay and South Africa.
细齿长蠕孢（Ophiostoma stenoceras）是一种常见的边材定植真菌，分布于北半球的一些针叶树和阔叶树上。在南半球，该真菌仅在新西兰有报道。有研究认为，人类病原体申克孢子丝菌（Sporothrix schenckii）是细齿长蠕孢的无性型。本研究旨在更好地了解这两个物种之间的系统发育关系。该研究还提供了一个机会，可以确认最近从世界各地的木材和土壤中收集到的一些申克孢子丝菌和类似细齿长蠕孢的分离物的身份。为此，确定了核糖体 RNA 基因座内部转录间隔区（ITS）区域的 DNA 序列。本研究包括形态上与细齿长蠕孢相似的 O. nigrocarpum、O. albidum、O. abietinum、O. narcissi 和 O. ponderosae 等种的菌株。从序列数据分析的系统发育树中，观察到四个主要的分支。这些分支代表了细齿长蠕孢、O. nigrocarpum 和两个包含申克孢子丝菌分离株的独立分支。我们的研究结果证实了之前的建议，即申克孢子丝菌应归入有性型 Ophiostoma 属，但支持将细齿长蠕孢和申克孢子丝菌分开的研究。O. albidum 和 O. ponderosae 应被视为细齿长蠕孢的同义词。O. narcissi 和 O. abietinum 的地位需要进一步澄清。申克孢子丝菌内的两个分支可能代表两个种，但这需要进一步确认。本研究首次报道了细齿长蠕孢在哥伦比亚、肯尼亚、乌拉圭和南非的分布。
