Anba J, Bidaud M, Vasil M L, Lazdunski A
Laboratoire de Chimie Bactérienne, C.N.R.S. Marseille, France.
J Bacteriol. 1990 Aug;172(8):4685-9. doi: 10.1128/jb.172.8.4685-4689.1990.
The nucleotide sequence of Pseudomonas aeruginosa phoB was determined. The sequence data suggest that the PhoB polypeptide consists of 229 amino acid residues and has a predicted molecular weight of 25,708. In the regulatory region of the gene, a very well conserved phosphate box was found. The sequence data also predicted the presence of an open reading frame downstream of phoB, which could be phoR. The deduced amino acid sequence of phoB was significantly homologous to that of the Escherichia coli phoB gene product and to those of several known procaryotic transcriptional regulators such as PhoP, OmpR, VirG, Dye, NtrC, and AlgR.
测定了铜绿假单胞菌phoB的核苷酸序列。序列数据表明,PhoB多肽由229个氨基酸残基组成,预测分子量为25708。在该基因的调控区域,发现了一个高度保守的磷酸盐盒。序列数据还预测在phoB下游存在一个开放阅读框,可能是phoR。推导的phoB氨基酸序列与大肠杆菌phoB基因产物以及几种已知的原核转录调节因子如PhoP、OmpR、VirG、Dye、NtrC和AlgR的序列具有显著同源性。