我们能否使用亚胺培南和美罗培南 Vitek 2 MIC 来检测肠杆菌科中疑似的 KPC 和其他碳青霉烯酶产生菌？
Can we use imipenem and meropenem Vitek 2 MICs for detection of suspected KPC and other-carbapenemase producers among species of Enterobacteriaceae?
机构信息
Servicio Antimicrobianos, Departamento Bacteriología, Instituto Nacional de Enfermedades Infecciosas (INEI)-ANLIS Carlos G. Malbrán, Ministerio de Salud y Ambiente de la Nación, Ciudad Autónoma de Buenos Aires, Argentina.
出版信息
J Clin Microbiol. 2011 Feb;49(2):697-701. doi: 10.1128/JCM.01178-10. Epub 2010 Dec 15.
Abstract
Imipenem and meropenem Vitek 2 MICs were evaluated for a panel of 104 Enterobacteriaceae for identification of carbapenemase producers. The sensitivity and specificity values for the new CLSI interpretative criteria (CLSI document M100-S20-U, 2010) were 98% and 83% for imipenem and 76% and 83% for meropenem, respectively. We propose an algorithm that is highly sensitive (98%) and specific (94%) for carbapenemase screening based on the combined use of imipenem and meropenem MICs.
摘要
对 104 株肠杆菌科细菌进行了亚胺培南和美罗培南 Vitek 2 MIC 值检测，以鉴定碳青霉烯酶产生菌。新 CLSI 解释标准（CLSI 文献 M100-S20-U，2010）对亚胺培南的敏感性和特异性值分别为 98%和 83%，对美罗培南的敏感性和特异性值分别为 76%和 83%。我们提出了一种基于联合使用亚胺培南和美罗培南 MIC 值的碳青霉烯酶筛选算法，该算法具有高敏感性（98%）和特异性（94%）。
相似文献
1
Can we use imipenem and meropenem Vitek 2 MICs for detection of suspected KPC and other-carbapenemase producers among species of Enterobacteriaceae?我们能否使用亚胺培南和美罗培南 Vitek 2 MIC 来检测肠杆菌科中疑似的 KPC 和其他碳青霉烯酶产生菌？
J Clin Microbiol. 2011 Feb;49(2):697-701. doi: 10.1128/JCM.01178-10. Epub 2010 Dec 15.
2
Comparative evaluation of combined-disk tests using different boronic acid compounds for detection of klebsiella pneumoniae carbapenemase-producing enterobacteriaceae clinical isolates.不同硼酸化合物联合碟试验检测产碳青霉烯酶肠杆菌科临床分离株的比较评价。
J Clin Microbiol. 2011 Aug;49(8):2804-9. doi: 10.1128/JCM.00666-11. Epub 2011 Jun 1.
3
Comparison of imipenem and meropenem antibiotics for the MALDI-TOF MS detection of carbapenemase activity.亚胺培南和美罗培南抗生素用于基质辅助激光解吸电离飞行时间质谱检测碳青霉烯酶活性的比较。
J Microbiol Methods. 2017 Jun;137:30-33. doi: 10.1016/j.mimet.2017.04.003. Epub 2017 Apr 5.
4
Differences between meropenem and imipenem disk to detect carbapenemase in gram-negative bacilli using simplified carbapenem inactivation method.使用简化的碳青霉烯失活法检测革兰氏阴性杆菌中产碳青霉烯酶时，美罗培南和亚胺培南药敏纸片的差异。
J Infect Chemother. 2020 Jun;26(6):636-639. doi: 10.1016/j.jiac.2020.02.012. Epub 2020 Mar 12.
5
Development of an algorithm for phenotypic screening of carbapenemase-producing Enterobacteriaceae in the routine laboratory.常规实验室中用于产碳青霉烯酶肠杆菌科细菌表型筛选算法的开发。
BMC Infect Dis. 2017 Jan 17;17(1):78. doi: 10.1186/s12879-016-2174-y.
6
Evaluation of the vitek 2 AST-N269 card for detection of meropenem resistance in imipenem-susceptible meropenem-resistant Enterobacteriaceae.评估Vitek 2 AST-N269卡片检测对亚胺培南敏感但对美罗培南耐药的肠杆菌科细菌中耐美罗培南情况的能力。
J Clin Microbiol. 2013 Nov;51(11):3908. doi: 10.1128/JCM.02063-13. Epub 2013 Aug 21.
7
Modified Hodge test using Mueller-Hinton agar supplemented with cloxacillin improves screening for carbapenemase-producing clinical isolates of Enterobacteriaceae.使用添加氯唑西林的穆勒-欣顿琼脂进行改良 Hodge 试验可提高对产碳青霉烯酶的肠杆菌科临床分离株的筛查。
J Med Microbiol. 2015 Jul;64(7):774-777. doi: 10.1099/jmm.0.000068. Epub 2015 May 1.
8
Comparison of disk diffusion, Etest and VITEK2 for detection of carbapenemase-producing Klebsiella pneumoniae with the EUCAST and CLSI breakpoint systems.比较纸片扩散法、Etest 和 VITEK2 检测产碳青霉烯酶肺炎克雷伯菌的性能，以 EUCAST 和 CLSI 折点系统为标准。
Clin Microbiol Infect. 2011 May;17(5):668-74. doi: 10.1111/j.1469-0691.2010.03299.x.
9
[Comparison of the modified Hodge test and the Carba NP test for detection of carbapenemases in Enterobacteriaceae isolates].[改良 Hodge 试验与 Carba NP 试验用于检测肠杆菌科分离株中碳青霉烯酶的比较]
Mikrobiyol Bul. 2016 Jan;50(1):1-10. doi: 10.5578/mb.10861.
10
Rapid and accurate detection of carbapenemase genes in Enterobacteriaceae with the Cepheid Xpert Carba-R assay.采用赛沛Xpert Carba-R检测法快速准确检测肠杆菌科细菌中的碳青霉烯酶基因。
J Med Microbiol. 2016 Sep;65(9):951-953. doi: 10.1099/jmm.0.000310. Epub 2016 Jul 19.
引用本文的文献
1
Performance of disk diffusion, gradient test, and VITEK 2 for carbapenem susceptibility testing in OXA-48-like carbapenemase-producing : a comparative study.纸片扩散法、梯度试验和VITEK 2在检测产OXA-48样碳青霉烯酶菌株对碳青霉烯类药物敏感性方面的性能：一项比较研究。
J Clin Microbiol. 2025 May 14;63(5):e0189324. doi: 10.1128/jcm.01893-24. Epub 2025 Apr 16.
2
Carbapenem-Resistant : Microbiology Key Points for Clinical Practice.耐碳青霉烯类：临床实践的微生物学要点
Int J Gen Med. 2019 Nov 28;12:437-446. doi: 10.2147/IJGM.S214305. eCollection 2019.
3
A locked nucleic acid (LNA)-based real-time PCR assay for the rapid detection of multiple bacterial antibiotic resistance genes directly from positive blood culture.一种基于锁核酸（LNA）的实时PCR检测方法，用于直接从阳性血培养物中快速检测多种细菌抗生素耐药基因。
PLoS One. 2015 Mar 16;10(3):e0120464. doi: 10.1371/journal.pone.0120464. eCollection 2015.
4
Evaluation of carbapenemase screening and confirmation tests with Enterobacteriaceae and development of a practical diagnostic algorithm.肠杆菌科细菌碳青霉烯酶筛查和确认试验的评估及实用诊断算法的开发
J Clin Microbiol. 2015 Jan;53(1):95-104. doi: 10.1128/JCM.01692-14. Epub 2014 Oct 29.
5
Carbapenemases in Klebsiella pneumoniae and other Enterobacteriaceae: an evolving crisis of global dimensions.肺炎克雷伯菌和其他肠杆菌科细菌中的碳青霉烯酶：具有全球范围的不断演变的危机。
Clin Microbiol Rev. 2012 Oct;25(4):682-707. doi: 10.1128/CMR.05035-11.
6
Characterization of carbapenem-nonsusceptible Klebsiella pneumoniae bloodstream isolates at a Taiwanese hospital: clinical impacts of lowered breakpoints for carbapenems.中文译文：台湾某医院耐碳青霉烯类肺炎克雷伯菌血流感染分离株的特征：碳青霉烯类药物折点降低的临床影响。
Eur J Clin Microbiol Infect Dis. 2012 Aug;31(8):1941-50. doi: 10.1007/s10096-011-1525-2. Epub 2012 Jan 18.
7
Accuracy of carbapenem nonsusceptibility for identification of KPC-possessing Enterobacteriaceae by use of the revised CLSI breakpoints.修订版 CLSI 折点用于检测携带 KPC 肠杆菌科细菌时，碳青霉烯类药物不敏感的准确性。
J Clin Microbiol. 2011 Nov;49(11):3931-3. doi: 10.1128/JCM.01176-11. Epub 2011 Aug 31.
本文引用的文献
1
Extended-spectrum [beta]-lactamase producing Escherichia coli: changing epidemiology and clinical impact.产超广谱β-内酰胺酶大肠埃希菌：不断变化的流行病学和临床影响。
Curr Opin Infect Dis. 2010 Aug;23(4):320-6. doi: 10.1097/qco.0b013e3283398dc1.
2
A sensitive and specific phenotypic assay for detection of metallo-β-lactamases and KPC in Klebsiella pneumoniae with the use of meropenem disks supplemented with aminophenylboronic acid, dipicolinic acid and cloxacillin.一种利用美罗培南纸片补充氨苯硼酸、二吡啶甲酸和氯唑西林检测肺炎克雷伯菌中产金属β-内酰胺酶和 KPC 的敏感、特异表型检测方法。
Clin Microbiol Infect. 2011 Apr;17(4):552-6. doi: 10.1111/j.1469-0691.2010.03294.x.
3
Escherichia coli sequence type ST131 as the major cause of serious multidrug-resistant E. coli infections in the United States.大肠杆菌序列型 ST131 是导致美国严重的多药耐药大肠杆菌感染的主要原因。
Clin Infect Dis. 2010 Aug 1;51(3):286-94. doi: 10.1086/653932.
4
Comparison of BD Phoenix, Vitek 2, and MicroScan automated systems for detection and inference of mechanisms responsible for carbapenem resistance in Enterobacteriaceae.比较 BD Phoenix、Vitek 2 和 MicroScan 自动化系统在检测和推断肠杆菌科碳青霉烯类耐药机制中的作用。
J Clin Microbiol. 2010 Aug;48(8):2999-3002. doi: 10.1128/JCM.00341-10. Epub 2010 Jun 9.
5
Comparison of meropenem MICs and susceptibilities for carbapenemase-producing Klebsiella pneumoniae isolates by various testing methods.不同检测方法检测产碳青霉烯酶肺炎克雷伯菌分离株的美罗培南 MIC 值和药敏结果比较。
J Clin Microbiol. 2010 Jul;48(7):2402-6. doi: 10.1128/JCM.00267-10. Epub 2010 May 19.
6
Controlling false-positive results obtained with the Hodge and Masuda assays for detection of class a carbapenemase in species of enterobacteriaceae by incorporating boronic Acid.通过加入硼酸来控制霍奇和増田检测肠杆菌科细菌 A 类碳青霉烯酶时产生的假阳性结果。
J Clin Microbiol. 2010 Apr;48(4):1323-32. doi: 10.1128/JCM.01771-09. Epub 2010 Feb 24.
7
Infections with extended-spectrum beta-lactamase-producing enterobacteriaceae: changing epidemiology and drug treatment choices.产超广谱β-内酰胺酶肠杆菌科感染：不断变化的流行病学和药物治疗选择。
Drugs. 2010 Feb 12;70(3):313-33. doi: 10.2165/11533040-000000000-00000.
8
Acquired carbapenemases in Gram-negative bacterial pathogens: detection and surveillance issues.革兰氏阴性细菌病原体中获得性碳青霉烯酶：检测和监测问题。
Clin Microbiol Infect. 2010 Feb;16(2):112-22. doi: 10.1111/j.1469-0691.2009.03116.x.
9
Controlling the spread of carbapenemase-producing Gram-negatives: therapeutic approach and infection control.控制产碳青霉烯酶革兰氏阴性菌的传播：治疗方法和感染控制。
Clin Microbiol Infect. 2010 Feb;16(2):102-11. doi: 10.1111/j.1469-0691.2009.03115.x.
10
Updated functional classification of beta-lactamases.β-内酰胺酶的功能分类更新。
Antimicrob Agents Chemother. 2010 Mar;54(3):969-76. doi: 10.1128/AAC.01009-09. Epub 2009 Dec 7.
Zotero 插件