Department of Clinical Laboratory, Wuhan Fourth Hospital, Puai Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, China.
Department of Pathophysiology, Key Laboratory of Ministry of Education for Neurological Disorders, School of Basic Medicine, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, China.
J Infect Chemother. 2020 Jun;26(6):636-639. doi: 10.1016/j.jiac.2020.02.012. Epub 2020 Mar 12.
The spread of carbapenemase-producing Enterobacteriaceae (CPE) is a major threat to public health. In the present study, we compared the difference between meropenem and imipenem disk for detecting carbapenemase-producing gram-negative bacilli using simplified carbapenem inactivation method (sCIM). 106 Enterobacteriaceae, including 74 CPE, 17 Pseudomonas aeruginosa including 10 carbapenemase-producing isolates and 36 Acinetobacter baumannii including 20 carbapenem-resistant isolates preserved in our laboratory were tested. Based on sCIM method, the test bacteria were tested with both meropenem and imipenem disk, respectively. In Enterobacteriaceae, the usage of both meropenem and imipenem disk showed high concordance (99.1%). Meropenem disk cannot identify positive isolates among the 10 P. aeruginosa and 20 A. baumannii isolates due to low carbapenem hydrolytic ability of the carbapenemase produced by these strains. Thus, meropenem disk was found to be similar to imipenem disk, presenting high specificity and sensitivity in the detection of carbapenemase in Enterobacteriaceae, but it cannot be used for the detection of carbapenemase in P. aeruginosa and A. baumannii.
碳青霉烯酶产生肠杆菌科（CPE）的传播对公共健康构成了重大威胁。在本研究中，我们使用简化的碳青霉烯失活法（sCIM）比较了美罗培南和亚胺培南纸片检测产碳青霉烯酶革兰氏阴性杆菌的差异。我们实验室保存了 106 株肠杆菌科，包括 74 株 CPE、17 株铜绿假单胞菌，包括 10 株产碳青霉烯酶的分离株和 36 株鲍曼不动杆菌，包括 20 株耐碳青霉烯的分离株。根据 sCIM 方法，分别用美罗培南和亚胺培南纸片检测试验菌。在肠杆菌科中，美罗培南和亚胺培南纸片的使用具有高度一致性（99.1%）。由于这些菌株产生的碳青霉烯酶对碳青霉烯的水解能力较低，美罗培南纸片不能识别 10 株铜绿假单胞菌和 20 株鲍曼不动杆菌分离株中的阳性分离株。因此，美罗培南纸片与亚胺培南纸片相似，在检测肠杆菌科中的碳青霉烯酶时具有高特异性和敏感性，但不能用于检测铜绿假单胞菌和鲍曼不动杆菌中的碳青霉烯酶。
