Department of Physics, Ryerson University, Toronto, Ontario M5B 2K3, Canada.
Phys Med Biol. 2011 Jan 21;56(2):315-26. doi: 10.1088/0031-9155/56/2/002. Epub 2010 Dec 15.
The aim of this study is to determine the impact of longitudinal micro-CT imaging on the growth of B16F1 tumours in C57BL/6 mice. Sixty mice received 2 × 10(5) B16F1 cells subcutaneously in the hind flank and were divided into control (no scan), 'low-dose' (80 kVp, 70 mA, 8 s, 0.07 Gy), 'medium-dose' (80 kVp, 50 mA, 30 s, 0.18 Gy) and 'high-dose' (80 kVp, 50 mA, 50 s, 0.30 Gy) groups. All imaging was performed on a fast volumetric micro-CT scanner (GE Locus Ultra, London, Canada). Each mouse was imaged on days 4, 8, 12 and 16. After the final imaging session, each tumour was excised, weighed on an electronic balance, imaged to obtain the final tumour volume and processed for histology. Final tumour volume was used to evaluate the impact of longitudinal micro-CT imaging on the tumour growth. An ANOVA indicated no statistically significant difference in tumour volume (p = 0.331, α = β = 0.1) when discriminating against a treatment-sized effect. Histological samples revealed no observable differences in apoptosis or cell proliferation. We conclude that four imaging sessions, using standard protocols, over the course of 16 days did not cause significant changes in final tumour volume for B16F1 tumours in female C57BL/6 mice (ANOVA, α = β = 0.1, p = 0.331).
本研究旨在确定纵向 micro-CT 成像对 C57BL/6 小鼠皮下 B16F1 肿瘤生长的影响。60 只小鼠在后侧腰部接受 2×10(5) B16F1 细胞皮下注射，并分为对照组（无扫描）、“低剂量”组（80 kVp、70 mA、8 s、0.07 Gy）、“中剂量”组（80 kVp、50 mA、30 s、0.18 Gy）和“高剂量”组（80 kVp、50 mA、50 s、0.30 Gy）。所有成像均在快速容积 micro-CT 扫描仪（GE Locus Ultra，加拿大伦敦）上进行。每只小鼠在第 4、8、12 和 16 天进行成像。最后一次成像后，切除每个肿瘤，在电子天平上称重，对其进行成像以获得最终肿瘤体积，并进行组织学处理。最终肿瘤体积用于评估纵向 micro-CT 成像对肿瘤生长的影响。方差分析表明，当区分治疗效果时，肿瘤体积无统计学差异（p = 0.331，α = β = 0.1）。组织学样本显示细胞凋亡或增殖无明显差异。我们得出结论，在 16 天的时间内进行四次成像，使用标准方案，不会导致雌性 C57BL/6 小鼠 B16F1 肿瘤的最终肿瘤体积发生显著变化（方差分析，α = β = 0.1，p = 0.331）。
