急性胆管炎时大鼠肝脏中MD-2和MD-2B表达的意义

Significance of MD-2 and MD-2B expression in rat liver during acute cholangitis.

作者信息

Miao Hui-Lai, Qiu Zhi-Dong, Hao Fu-Long, Bi Yong-Hui, Li Ming-Yi, Chen Ming, Chen Nian-Ping, Zhou Fei

机构信息

Hui-Lai Miao, Zhi-Dong Qiu, Ming-Yi Li, Ming Chen, Nian-Ping Chen, Department of Hepatobiliary Surgery, the Affiliated Hospital of Guangdong Medical College, Zhanjing 524001, Guangdong Province, China.

出版信息

World J Hepatol. 2010 Jun 27;2(6):233-8. doi: 10.4254/wjh.v2.i6.233.

DOI:10.4254/wjh.v2.i6.233

PMID:21161002

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC2999288/

Abstract

AIM

To investigate the expression of myeloid differentiation protein-2 (MD-2), MD-2B (a splicing isoform of MD-2 that can block Toll-like receptor 4 (TLR4)/MD-2 LPS-mediated signal transduction) and TLR4 in the liver of acute cholangitis rats.

METHODS

Male Sprague-Dawley rats (SPF level) were randomly divided into four groups: (A) sham-operated group; (B) simple common bile duct ligation group; (C) acute cholangitis group; and (D) acute cholangitis anti-TLR4 intervention group (n = 25 per group). Rat liver tissue samples were used to detect TLR4, MD-2 and MD-2B mRNA expression by fluorescence quantitative PCR in parallel with pathological changes.

RESULTS

In acute cholangitis, liver TLR4 and MD-2 mRNA expression levels at 6, 12, 24, 48 and 72 h were gradually up-regulated but MD-2B mRNA expression gradually down-regulated (P < 0.05). After TLR4 antibody treatment, TLR4 and MD-2 mRNA expression were lower compared with the acute cholangitis group (P < 0.05). However, MD-2B mRNA expression was higher than in the acute cholangitis group (P < 0.05). MD-2 and TLR4 mRNA expressions were positively correlated (r = 0.94981, P < 0.05) and MD-2B mRNA expression was negatively correlated with MD-2 and TLR4 mRNA (r = -0.89031, -0.88997, P < 0.05).

CONCLUSION

In acute cholangitis, MD-2 plays an important role in the process of TLR4- mediated inflammatory response to liver injury while MD-2B plays a negative regulatory role.

摘要

目的

研究急性胆管炎大鼠肝脏中髓样分化蛋白2（MD-2）、MD-2B（MD-2的一种剪接异构体，可阻断Toll样受体4（TLR4）/MD-2脂多糖介导的信号转导）及TLR4的表达。

方法

将雄性Sprague-Dawley大鼠（无特定病原体级）随机分为四组：（A）假手术组；（B）单纯胆总管结扎组；（C）急性胆管炎组；（D）急性胆管炎抗TLR4干预组（每组n = 25）。取大鼠肝脏组织样本，采用荧光定量PCR检测TLR4、MD-2和MD-2B mRNA表达，并观察病理变化。

结果

急性胆管炎时，6、12、24、48和72 h肝脏TLR4和MD-2 mRNA表达水平逐渐上调，但MD-2B mRNA表达逐渐下调（P < 0.05）。TLR4抗体处理后，与急性胆管炎组相比，TLR4和MD-2 mRNA表达降低（P < 0.05）。然而，MD-2B mRNA表达高于急性胆管炎组（P < 0.05）。MD-2与TLR4 mRNA表达呈正相关（r = 0.94981，P < 0.05），MD-2B mRNA表达与MD-2和TLR4 mRNA呈负相关（r = -0.89031，-0.88997，P < 0.05）。