[Effect of Salvia Miltiorrhiza Bunge on expression of osteoprotegerin in cultured human periodontal ligament fibroblasts].

PURPOSE

To study the effect of Salvia Miltiorrhiza Bunge on the expression of osteoprotegerin (OPG) in cultured human periodontal ligament fibroblasts.

METHODS

Primary culture of human periodontal ligament fibroblasts was established and the fifth passage cells were used in this study. Concentration-dependent effect of Salvia Miltiorrhiza Bunge on OPG mRNA and OPG protein secretion were determined by reverse transcription-polymerase chain reaction (RT-PCR) and enzyme linked immunosorbant assay (ELISA), respectively. The data were analyzed with SPSS15.0 software package.

RESULTS

OPG mRNA expression was increased after 1 h exposure to various concentrations of Salvia Miltiorrhiza Bunge compared with the control group (P<0.05). OPG protein secretion was significantly increased after 12 h exposure to various concentrations of Salvia Miltiorrhiza Bunge compared with the control group (P<0.05).

CONCLUSION

Salvia Miltiorrhiza Bunge can up-regulate the expression of OPG in cultured human periodontal ligament fibroblasts.