Yue Ma, Aishu Ren, Gang Fu
Dept. of Orthodontics, Stomatological Hospital of Chongqing Medical University, Chongqing 401147, China;Chongqing Key Laboratory of Oral Diseases and Biomedical Sciences, Chongqing 401147, China.
Chongqing Municipal Key Laboratory of Oral Biomedical Engineering of Higher Education, Chongqing 401147, China.
Hua Xi Kou Qiang Yi Xue Za Zhi. 2016 Oct 1;34(5):468-473. doi: 10.7518/hxkq.2016.05.007.
This study investigated the effects of salvianolic acid B (Sal B), a major bioactive component of the Chinese medicine salvia miltiorrhiza, on osteogenic differentiation of human periodontal ligament cells (hPDLCs).
Third passage PDLCs were used in this experiment. Methyl thiazolyl tetrazolium (MTT) method was employed to observe the effects of different Sal B concentrations on proliferation activity of hPDLCs. Alkaline phosphatase (ALP) activity and mineralization capability were measured, and mRNA expression of osteocalcin (OCN) was detected to investigate the effects of Sal B on osteogenesis of hPDLCs.
Sal B did not influence the viability of hPDLCs. The ALP activity and OCN mRNA expression levels of hPDLCs were both significantly improved (P<0.05) under treatment with different Sal B concentrations (0.5, 1, and 5 μmol·L⁻¹) compared with those in OIM group. Moreover, the number of mineralized nodules formed by hPDLCs were considerably higher under treatment with different Sal B concentrations (0.5, 1, and 5 μmol·L⁻¹) than that in the OIM group.
Appropriate Sal B concentration can improve the osteogenic differentiation of hPDLCs.
本研究探讨中药丹参的主要生物活性成分丹酚酸B（Sal B）对人牙周膜细胞（hPDLCs）成骨分化的影响。
本实验采用第三代PDLCs。采用甲基噻唑基四氮唑（MTT）法观察不同浓度Sal B对hPDLCs增殖活性的影响。检测碱性磷酸酶（ALP）活性和矿化能力，并检测骨钙素（OCN）的mRNA表达，以研究Sal B对hPDLCs成骨的影响。
Sal B不影响hPDLCs的活力。与OIM组相比，不同浓度Sal B（0.5、1和5μmol·L⁻¹）处理下hPDLCs的ALP活性和OCN mRNA表达水平均显著提高（P<0.05）。此外，不同浓度Sal B（0.5、1和5μmol·L⁻¹）处理下hPDLCs形成的矿化结节数量明显高于OIM组。
适当浓度的Sal B可促进hPDLCs的成骨分化。
