College of Chemistry and Chemical Engineering and State Key Laboratory of Applied Organic Chemistry, Lanzhou University, Lanzhou 730000, People's Republic of China.
J Fluoresc. 2011 May;21(3):1091-102. doi: 10.1007/s10895-010-0782-2. Epub 2010 Dec 15.
A novel Schiff base ligand, chromone-3-carbaldehyde-aminophenazone (L) and its Ln(III) (Ln = La, Yb) complexes were synthesized and characterized by physicochemical methods. The interaction between the ligand, Ln(III) complexes and calf thymus DNA in physiological buffer (pH=7.10) was investigated by using UV-vis spectroscopy, fluorescence spectra, ethidium bromide experiments and viscosity measurements, indicating that the studied compounds can all bind to DNA via an intercalation binding mode and the complexes have stronger binding affinity than the free ligand alone. Furthermore, antioxidant activity of the ligand and its complexes was determined by superoxide and hydroxyl radical scavenging methods in vitro, suggesting that Ln(III) complexes inhibit stronger antioxidant activity than the ligand alone and some standard antioxidants, such as mannitol and vitamin C.
一种新型的希夫碱配体，色酮-3-甲醛-氨基苯并恶嗪（L）及其镧（III）（Ln = La，Yb）配合物通过物理化学方法合成并进行了表征。通过紫外可见光谱、荧光光谱、溴化乙锭实验和粘度测量，研究了配体、Ln（III）配合物与小牛胸腺 DNA 在生理缓冲液（pH = 7.10）中的相互作用，表明所研究的化合物都可以通过嵌入结合模式与 DNA 结合，并且配合物比游离配体具有更强的结合亲和力。此外，通过超氧阴离子和羟基自由基清除方法在体外测定了配体及其配合物的抗氧化活性，表明 Ln（III）配合物的抑制抗氧化活性强于单独的配体，以及一些标准的抗氧化剂，如甘露醇和维生素 C。
