Institute of Microbiology, Chinese Academy of Sciences, Beijing, P R China.
Chem Biodivers. 2010 Dec;7(12):2901-7. doi: 10.1002/cbdv.200900349.
Two new resorcinol derivatives, 4-hydroxy-2-methoxy-6-[(8Z)-pentadec-8-en-1-yl]phenyl acetate (1) and 4-hydroxy-2-methoxy-6-pentadecylphenyl acetate (2), together with known compounds ardisiphenol D (3), 5-tridecylresorcinol (4), 5-pentadecylresorcinol (5), 5-[(8Z)-pentadec-8-en-1-yl]resorcinol (6), belamcandaquinones C and D (7 and 8, resp.), ardisicrenoside A, ardisiacrispin B, (22E)-24-ethyl-5α-cholesta-7,22-dien-3-one, and (22E)-24-ethyl-5α-cholesta-7,22-dien-3β-ol were isolated from the MeOH extract of the roots of Ardisia brevicaulis Diels. Their structures were determined by spectroscopic analysis including ESI- and EI-MS, and NMR data. Cytotoxicities of 1-4 against cell lines A549, MCF-7, and PANC-1 were tested in vitro by the MTT (=3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl-2H-tetrazolium bromide) method. Compounds 1-4 showed cytotoxic activity against all cell lines stronger than that of cisplatin against A549.
从短葶山麦冬的根的甲醇提取物中分离得到了两个新的间苯二酚衍生物，4-羟基-2-甲氧基-6-[(8Z)-十五-8-烯-1-基]苯基乙酸酯（1）和 4-羟基-2-甲氧基-6-十五烷基苯基乙酸酯（2），以及已知化合物 Ardisiaphenol D（3）、5-十三烷基间苯二酚（4）、5-十五烷基间苯二酚（5）、5-[(8Z)-十五-8-烯-1-基]间苯二酚（6）、Belamcandaquinones C 和 D（7 和 8）、ardisicrenoside A、ardisiacrispin B、(22E)-24-乙基-5α-胆甾-7,22-二烯-3-酮和(22E)-24-乙基-5α-胆甾-7,22-二烯-3β-醇。通过包括 ESI-和 EI-MS 以及 NMR 数据在内的光谱分析确定了它们的结构。通过 MTT（=3-(4,5-二甲基噻唑-2-基)-2,5-二苯基-2H-四唑溴盐）法测定了化合物 1-4 对 A549、MCF-7 和 PANC-1 细胞系的体外细胞毒性。化合物 1-4 对所有细胞系的细胞毒性均强于顺铂对 A549 的细胞毒性。
