Wang Xu-Guang, Zhang Zhong, Sun Li-Ping, Zhang Ye, Yuan Yuan
Cancer Control Laboratory of Cancer Institute and General Surgery, the First Affiliated Hospital of China Medical University, Shenyang 110001, China; Department of Pathology, Shenyang Medical Collage.
Zhonghua Liu Xing Bing Xue Za Zhi. 2010 Aug;31(8):920-4.
To detect the distribution of polymorphism of GSTP1 and infection rate of Helicobacter pylori (H. pylori) in different kinds of gastric intestinal metaplasia (IM) and to explore the relationship between entergenic factor-polymorphism of GSTP1 and extogenic factor-H. pylori infection, to determine the risk of subtype of IM.
There were 381 cases in total, including 143 CGS(+) and 238 IM. H. E. stain was used for pathological diagnosis. HID-AB-pH2.5 methods were used to classify the IM. ELISA method was used to detect the antibody of H. pylor-IgG. Polymerase chain reaction (PCR) and restriction fragment length polymorphism (RFLP) were used to analyze the genotype.
Frequency of G genotype was higher in the IM group, when compared to the CGS(+) groups (P < 0.05). The positive rate of H. pylori was statistically higher than CGS(+) (P < 0.01). By effect-modified model analysis, GSTP1 gene polymorphsim and H. pylori infection presented a positive interaction in the stage IM, with the OR value as 9.386 (95%CI: 3.736 - 23.580) after adjusted by age and gender. The synergy index was 2.078 and the attributable proportion of interaction was 46.36%. The positive rate of H. pylori were statistically highter than CGS(+) group in subtype IMI, subtype IMII and III (P < 0.01). The frequency of G genotype was higher in the IMII and III group, when compared with the IMI groups (P < 0.01). By effect-modified model analysis, in the stage of IMII and III, GSTP1 gene polymorphsim and H. pylori infection also presented a positive interaction, with OR value as 24.487 (95%CI: 7.731 - 77.735) after adjusted by age and gender, with its synergy index as 1.844, and attributable proportion of interaction as 43.89%.
The infection of H. pylori and polymorphism GSTP1gene appeared to be both the external and internal factors, respectively. In the stage of IM, GSTP1 gene polymorphsim and H. pylori infection also presented a positive interaction, expecially in the IMII and III.
检测不同类型胃肠化生（IM）中谷胱甘肽S转移酶P1（GSTP1）基因多态性分布及幽门螺杆菌（H. pylori）感染率，探讨内源性因素-GSTP1基因多态性与外源性因素-H. pylori感染之间的关系，确定IM各亚型的风险。
共381例，其中143例为慢性浅表性胃炎（CGS(+)），238例为IM。采用苏木精-伊红（HE）染色进行病理诊断。采用高铁二胺-阿尔辛蓝-pH2.5法对IM进行分型。采用酶联免疫吸附测定（ELISA）法检测幽门螺杆菌IgG抗体。采用聚合酶链反应（PCR）和限制性片段长度多态性（RFLP）分析基因型。
与CGS(+)组相比，IM组G基因型频率更高（P < 0.05）。幽门螺杆菌阳性率在统计学上高于CGS(+)组（P < 0.01）。通过效应修正模型分析，在IM阶段，GSTP1基因多态性与幽门螺杆菌感染呈现正交互作用，经年龄和性别校正后，比值比（OR）值为9.386（95%可信区间：3.736 - 23.580）。协同指数为2.078，交互作用归因比例为46.36%。在IMI、IMII和III亚型中，幽门螺杆菌阳性率在统计学上高于CGS(+)组（P < 0.01）。与IMI组相比，IMII和III组G基因型频率更高（P < 0.01）。通过效应修正模型分析，在IMII和III阶段，GSTP1基因多态性与幽门螺杆菌感染也呈现正交互作用，经年龄和性别校正后，OR值为24.487（95%可信区间：7.731 - 77.735），协同指数为1.844，交互作用归因比例为43.89%。
幽门螺杆菌感染和GSTP1基因多态性似乎分别是外源性和内源性因素。在IM阶段，GSTP1基因多态性与幽门螺杆菌感染也呈现正交互作用，尤其是在IMII和III型中。
