Yu Jin-ning, Li Hao, Tang Qing, Tao Xiao-yan, Wu Hui, Mo Zhao-jun, Zhang Hong, Wang Ding-ming, Weng Jing-qing, Shen Rui-hua, Zhu Feng-cai, Wang Xian-jun, Liu Hong, Shen Xin-xin, Wang Shu-mei
State Key Laboratory for Molecular Virology and Genetic Engineering, National Institute for Viral Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing 100052, China.
Zhonghua Liu Xing Bing Xue Za Zhi. 2010 May;31(5):521-4.
To investigate the status of infection and distribution of rabies virus (RV) in different epidemic areas in China.
Brain specimens from animals and suspected patients were collected at the districts of high-, medium- and low incidence rates of human rabies and detected by both direct Immunofluorescence assay (DFA) and RT-PCR.
254 of 3007 specimens of dog brains showed RV positive by DFA (positive rate of 8.4%). Among these 254 samples, 78 showed positive (positive rate of 30.7%) by RT-PCR. 93 specimens from dogs and cats that had attacked human beings, 63 of them showed positive by DFA (positive rate of 67.7%) and all of them were also positive by RT-PCR. In addition, RV could also be detected in Apodemus agrarius, ferret badger, and suspected patients specimens from the districts under survey. There was no statistical difference between the infection rates of RV in different provinces and regions with different incidence of rabies.
There might be a relatively high infection rate of RV among the domestic dogs/cats in the endemic areas in China. Wild animals might have been infected with RV in the districts under survey.
调查中国不同疫区狂犬病病毒（RV）的感染状况及分布情况。
在人间狂犬病高、中、低发病率地区采集动物及疑似患者的脑标本，采用直接免疫荧光法（DFA）和逆转录聚合酶链反应（RT-PCR）进行检测。
3007份犬脑标本中，254份经DFA检测显示RV阳性（阳性率8.4%）。在这254份样本中，78份经RT-PCR检测呈阳性（阳性率30.7%）。93份来自袭击过人的犬和猫的标本，其中63份经DFA检测呈阳性（阳性率67.7%），且所有这些标本经RT-PCR检测也均为阳性。此外，在所调查地区的黑线姬鼠、鼬獾及疑似患者标本中也能检测到RV。不同狂犬病发病率的省份和地区之间，RV的感染率无统计学差异。
中国狂犬病疫区家犬/猫中RV感染率可能较高。在所调查地区野生动物可能已感染RV。
