Laboratory for Orthopaedic Pathophysiology and Regenerative Medicine, Istituto Ortopedico Rizzoli, Bologna, Italy.
Acta Biomater. 2011 Apr;7(4):1780-7. doi: 10.1016/j.actbio.2010.12.012. Epub 2010 Dec 14.
Despite their known osteoconductivity, clinical use of calcium phosphate cements is limited both by their relatively slow rate of resorption and by rheological properties incompatible with injectability. Bone in-growth and material resorption have been improved by the development of porous calcium phosphate cements. However, injectable formulations have so far only been obtained through the addition of relatively toxic surfactants. The present work describes the response of osteoblasts to a novel injectable foamed bone cement based on a composite formulation including the bioactive foaming agents soybean and gelatine. The foaming properties of both defatted soybean and gelatine gels were exploited to develop a self-hardening soy/gelatine/hydroxyapatite composite foam able to retain porosity upon injection. After setting, the foamed paste produced a calcium-deficient hydroxyapatite scaffold, showing good injectability and cohesion as well as interconnected porosity after injection. The intrinsic bioactivity of soybean and gelatine was shown to favour osteoblast adhesion and growth. These findings suggest that injectable, porous and bioactive calcium phosphate cements can be produced for bone regeneration through minimally invasive surgery.
尽管磷酸钙骨水泥具有已知的骨诱导性，但由于其吸收速度相对较慢，以及流变学性能与可注射性不兼容，其临床应用受到限制。通过开发多孔磷酸钙骨水泥，已经改善了骨向内生长和材料吸收。然而，可注射制剂迄今为止仅通过添加相对有毒的表面活性剂来获得。本工作描述了成骨细胞对新型可注射泡沫骨水泥的反应，该骨水泥基于包括生物活性发泡剂大豆和明胶的复合配方。利用脱脂大豆和明胶凝胶的发泡性能，开发了一种自固化的大豆/明胶/羟基磷灰石复合泡沫，能够在注射时保持孔隙率。凝固后，泡沫糊剂产生了一种缺钙羟基磷灰石支架，在注射后具有良好的可注射性和内聚性以及连通性孔隙。大豆和明胶的固有生物活性有利于成骨细胞的黏附和生长。这些发现表明，可通过微创外科手术生产用于骨再生的可注射、多孔和生物活性的磷酸钙骨水泥。
