Amaratunga K, Goodwin P M, O'Connor C D, Anthony C
Department of Biochemistry, School of Biological Sciences, University of Southampton, UK.
FEMS Microbiol Lett. 1997 Jan 1;146(1):31-8. doi: 10.1111/j.1574-6968.1997.tb10167.x.
MxaJ is a protein of unknown function encoded by mxaJ in the mxaFJGI operon. We have constructed a mxaJ mutant of M. extorquens with a deletion which does not affect transcription of downstream genes. It contained cytochrome cL (MxaG), but neither subunit of methanol dehydrogenase (MxaF and MxaI). MxaJ is probably involved in processing this enzyme. We have sequenced the region between mxaFJGI and five other methanol oxidation genes, mxaACKLD; it includes one open reading frame (mxaR) and a possible second open reading frame (mxaS), demonstrating the presence in M. extorquens of the following gene cluster: mxaFJGIR(S) ACKLD.
MxaJ是mxaFJGI操纵子中由mxaJ编码的一种功能未知的蛋白质。我们构建了扭脱甲基杆菌的mxaJ突变体，该突变体存在一个缺失，且该缺失不影响下游基因的转录。它含有细胞色素cL（MxaG），但不含有甲醇脱氢酶的任何一个亚基（MxaF和MxaI）。MxaJ可能参与该酶的加工过程。我们对mxaFJGI和其他五个甲醇氧化基因mxaACKLD之间的区域进行了测序；该区域包括一个开放阅读框（mxaR）和一个可能的第二个开放阅读框（mxaS），这表明扭脱甲基杆菌中存在以下基因簇：mxaFJGIR(S)ACKLD。
