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来自甲基营养型芽胞杆菌AM1的mxaA、mxaC、mxaK、mxaL和mxaD基因的鉴定及核苷酸序列

Identification and nucleotide sequences of mxaA, mxaC, mxaK, mxaL, and mxaD genes from Methylobacterium extorquens AM1.

作者信息

Morris C J, Kim Y M, Perkins K E, Lidstrom M E

机构信息

California Institute of Technology, Pasadena 91125, USA.

出版信息

J Bacteriol. 1995 Dec;177(23):6825-31. doi: 10.1128/jb.177.23.6825-6831.1995.

DOI:10.1128/jb.177.23.6825-6831.1995
PMID:7592474
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC177549/
Abstract

The DNA sequence for a 4.4-kb HindIII-XhoI Methylobacterium extorquens AM1 DNA fragment that is known to contain three genes (mxaAKL) involved in incorporation of calcium into methanol dehydrogenase (I. W. Richardson and C. Anthony, Biochem. J. 287:709-7115, 1992) was determined. Five complete open reading frames and two partial open reading frames were found, suggesting that this region contains previously unidentified genes. A combination of sequence analysis, mutant complementation data, and gene expression studies showed that these genes correspond to mxaSACKLDorf1. Of the three previously unidentified genes (mxaC, mxaD, and orf1), mutant complementation studies showed that mxaC is required for methanol oxidation, while the function of the other two genes is still unknown.

摘要

已测定了4.4kb的甲基营养型芽孢杆菌AM1 HindIII-XhoI DNA片段的DNA序列，已知该片段包含三个参与将钙掺入甲醇脱氢酶的基因（mxaAKL）（I. W. 理查森和C. 安东尼，《生物化学杂志》287:709 - 7115，1992）。发现了五个完整的开放阅读框和两个部分开放阅读框，这表明该区域包含以前未鉴定的基因。序列分析、突变体互补数据和基因表达研究的结合表明，这些基因对应于mxaSACKLDorf1。在三个以前未鉴定的基因（mxaC、mxaD和orf1）中，突变体互补研究表明mxaC是甲醇氧化所必需的，而其他两个基因的功能仍然未知。

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