Industrial Technology Center of Okayama Prefecture, 5301 Haga, Kita-ku, Okayama 701-1296, Japan.
J Biosci Bioeng. 2011 Mar;111(3):300-5. doi: 10.1016/j.jbiosc.2010.11.008. Epub 2010 Dec 16.
Solid-state fermentation (SSF) has attracted a lot of interest for carrying out high-level protein production in filamentous fungi. However, it has problems such as the fermentation heat generated during the culture in addition to the reduced mobility of substances. These conditions lead to a nonuniform state in the culture substrate and result in low reproducibility. We constructed a non-airflow box (NAB) with a moisture permeable fluoropolymer membrane, thereby making it possible to control and maintain uniform and optimal conditions in the substrate. For the NAB culture in Aspergillus oryzae, temperature and water content on/in the whole substrate were more consistent than for a traditional tray box (TB) culture. Total weight after the culture remained constant and dry conditions could be achieved during the culture. These data demonstrate the possibility of growing a uniform culture of the whole substrate for SSF. The NAB is advantageous because it allows for the control of exact temperature and water content in the substrate during the culture by allowing vapor with latent heat to dissipate out of the box. In addition, several enzymes in the NAB culture exhibited higher production levels than in the TB culture. We believe that culturing in the constructed NAB could become a standard technique for commercial SSF.
固态发酵（SSF）在丝状真菌中进行高水平蛋白质生产方面引起了广泛关注。然而，它存在一些问题，例如培养过程中产生的发酵热以及物质流动性降低。这些条件导致培养底物不均匀，重现性差。我们构建了一个具有透气透湿性氟聚合物膜的非气流箱（NAB），从而可以控制和维持底物中的均匀和最佳条件。与传统托盘箱（TB）培养相比，在米曲霉的 NAB 培养中，整个底物的温度和含水量更加一致。培养后的总重量保持不变，并且在培养过程中可以实现干燥条件。这些数据表明 SSF 可以实现整个底物均匀培养的可能性。NAB 的优势在于，它允许蒸汽携带潜热从箱内散发出去，从而可以在培养过程中控制底物中的确切温度和含水量。此外，NAB 培养中的几种酶的产量水平高于 TB 培养中的产量水平。我们相信，在构建的 NAB 中进行培养可以成为商业 SSF 的标准技术。
