Bernhard Nocht Institute for Tropical Medicine, Hamburg, Germany.
J Med Microbiol. 2011 Apr;60(Pt 4):454-458. doi: 10.1099/jmm.0.025338-0. Epub 2010 Dec 16.
Strongyloidiasis is a neglected disease that is prevalent mainly in tropical and subtropical regions. It is caused by intestinal nematodes of the genus Strongyloides. Due to the rise in worldwide travel, infections are increasingly encountered in non-endemic regions. Diagnosis is hampered by insensitive and laborious detection methods. A universal Strongyloides species real-time PCR was developed with an internal competitive control system. The 95% limit of detection as determined by probit analysis was one larva per PCR equivalent to 100 larvae per 200 mg stool. The assay proved to be 100% specific as assessed using a panel of parasites and bacteria and thus might be useful in the diagnostic setting as well as for Strongyloides research.
钩虫病是一种被忽视的疾病，主要流行于热带和亚热带地区。它是由肠道线虫属的钩虫引起的。由于全球旅行的增加，在非流行地区越来越多地发现感染。诊断受到不敏感和繁琐的检测方法的阻碍。开发了一种通用的钩虫属实时 PCR 检测方法，带有内部竞争控制系统。通过概率分析确定的 95%检测限为每 PCR 等效物 1 条幼虫，相当于每 200mg 粪便 100 条幼虫。该检测方法通过寄生虫和细菌的面板评估显示出 100%的特异性，因此可能在诊断环境以及钩虫研究中都有用。
