使用 IkappaB-荧光素酶替代标记测定法分析消耗的姜黄素类的尿液和血清。
Urine and serum analysis of consumed curcuminoids using an IkappaB-luciferase surrogate marker assay.
机构信息
800 Research Parkway Suite 350, Oklahoma City, OK, 73104, U.S.A.
出版信息
In Vivo. 2010 Nov-Dec;24(6):861-4.
BACKGROUND
curcumin metabolites are detectable in body fluids such as serum and urine. We have developed a novel assay that can detect metabolites in such body fluids by measuring their effect on the nuclear factor kappa B/inhibitor of kappa B (NF-κB/IκB) pathway.
PATIENTS AND METHODS
fifteen healthy individuals were enrolled in the study and randomly assigned to two groups: control group (five) and curcumin group (ten). The test group ingested 8 g of the curcuminoids (C(3)-Complex) with 16 oz of bottled water. Blood and urine were collected at 0, 4, 8, and 24 h after ingestion. Degradation of the NF-κB/IκB complex was detected by the Genetic Expression and Measurement (GEM) assay using HCT116 cells stably transfected with PGL3-IκB firefly luciferase.
RESULTS
using our novel GEM assay, the five controls who had not taken curcumin were identified.
CONCLUSION
the GEM assay is a very sensitive and accurate non-invasive assay that could be utilized to detect metabolites in body fluids. It could also serve as a tool to determine participants' compliance during clinical research studies.
背景
姜黄素代谢物可在血清和尿液等体液中检测到。我们开发了一种新的测定方法，通过测量其对核因子 kappa B/抑制物 kappa B（NF-κB/IκB）通路的影响，可检测此类体液中的代谢物。
患者和方法
本研究纳入 15 名健康个体，并将其随机分为两组：对照组（5 名）和姜黄素组（10 名）。实验组摄入 8 克姜黄素（C（3）-复合物），并用 16 盎司瓶装水送服。摄入后 0、4、8 和 24 小时采集血液和尿液。使用稳定转染 PGL3-IκB 萤火虫荧光素酶的 HCT116 细胞，通过遗传表达和测量（GEM）测定法检测 NF-κB/IκB 复合物的降解。
结果
使用我们的新型 GEM 测定法，鉴定出未服用姜黄素的 5 名对照者。
结论
GEM 测定法是一种非常敏感和准确的非侵入性测定法，可用于检测体液中的代谢物。它还可以作为一种工具，用于确定临床研究中参与者的依从性。
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