Department of Pathology, Brigham and Women's Hospital, Boston, MA 02115, USA.
Am J Surg Pathol. 2011 Jan;35(1):76-83. doi: 10.1097/PAS.0b013e31820065c9.
CD200, an immunoglobulin superfamily membrane glycoprotein, is expressed in B cells, a subset of T cells, and in a range of B-cell lymphoproliferative disorders. We recently found, by immunohistochemical staining, that follicular helper T cells associated with neoplastic L and H cells in nodular lymphocyte predominant Hodgkin lymphoma, express CD200. Here we show that CD200 is expressed by follicular helper T cells in reactive lymphoid tissue, using single-color and 2-color immunohistochemical staining. Immunohistochemical staining of a range of T-cell lymphoproliferative disorders shows that the neoplastic cells in angioimmunoblastic T-cell lymphoma are immunoreactive for CD200, and the pattern of expression is similar to that of other follicular helper T-cell markers, PD-1 and CXCL13. In contrast, only a minority of cases of T-cell neoplasms other than angioimmunoblastic T-cell lymphoma are immunoreactive for CD200. A subset of CD200-positive peripheral T-cell lymphoma, not otherwise specified, cases may represent evolving angioimmunoblastic T-cell lymphoma or another neoplasm derived from follicular T helper cells. We conclude that CD200 is a useful immunophenotypic marker of angioimmunoblastic T-cell lymphoma and may be a suitable therapeutic target for an anti-CD200 immunotherapy undergoing clinical trial.
CD200 是免疫球蛋白超家族的膜糖蛋白，在 B 细胞、T 细胞亚群以及一系列 B 细胞淋巴增生性疾病中表达。我们最近通过免疫组织化学染色发现，结节性淋巴细胞为主型霍奇金淋巴瘤中与肿瘤 L 和 H 细胞相关的滤泡辅助 T 细胞表达 CD200。在这里，我们使用单染和双色免疫组织化学染色显示，滤泡辅助 T 细胞在反应性淋巴组织中表达 CD200。对一系列 T 细胞淋巴增生性疾病的免疫组织化学染色显示，血管免疫母细胞性 T 细胞淋巴瘤中的肿瘤细胞对 CD200 呈免疫反应性，表达模式与其他滤泡辅助 T 细胞标志物 PD-1 和 CXCL13 相似。相比之下，除血管免疫母细胞性 T 细胞淋巴瘤以外的 T 细胞肿瘤仅有少数病例对 CD200 呈免疫反应性。一部分 CD200 阳性的外周 T 细胞淋巴瘤，非特指型，可能代表正在发展的血管免疫母细胞性 T 细胞淋巴瘤或另一种源自滤泡辅助 T 细胞的肿瘤。我们得出结论，CD200 是血管免疫母细胞性 T 细胞淋巴瘤的一个有用的免疫表型标志物，并且可能是正在进行临床试验的抗 CD200 免疫治疗的一个合适的治疗靶点。
