Penland R L, Wilhelmus K R
Sid W. Richardson Ocular Microbiology Laboratory, Cullen Eye Institute, Baylor College of Medicine, Houston, Texas, USA.
Am J Ophthalmol. 1998 Oct;126(4):590-2. doi: 10.1016/s0002-9394(98)00125-1.
To evaluate the use of buffered charcoal-yeast extract agar for the isolation of Acanthamoeba from clinical specimens.
We retrospectively reviewed laboratory records of patients with ocular acanthamebic infection from October 1993 to September 1997 to compare the recovery of Acanthamoeba from clinical specimens inoculated onto various media. We then compared the experimental recovery of 10 corneal isolates of Acanthamoeba on buffered charcoal-yeast extract and blood agars.
Paired data for buffered charcoal-yeast extract and blood agars were available from 24 cultures performed in 13 cases of ocular acanthamebic infection. Acanthamebic trails were detected on both buffered charcoal-yeast extract and blood agars in nine cultures, only on buffered charcoal-yeast extract agar in nine cultures, and only on blood agar in one culture (P = .027). In the experimental study, all 10 clinical isolates produced trails on buffered charcoal-yeast extract agar, and the mean recovery after 10 days of incubation ranged from 38% to 95% of the original inoculum number. For seven of the 10 isolates, more than 70% of the original inoculum was recovered on buffered charcoal-yeast extract agar. Only two of the 10 strains produced persistent trails on the blood agar, and the mean recoveries after 10 days of incubation were 0.67% and 1.17%. Recovery was significantly better on buffered charcoal-yeast extract agar than blood agar (P < or = .0003).
Buffered charcoal-yeast extract agar is an excellent commercially available culture medium for the recovery of Acanthamoeba.
评估使用缓冲炭酵母提取物琼脂从临床标本中分离棘阿米巴的效果。
我们回顾性分析了1993年10月至1997年9月眼部棘阿米巴感染患者的实验室记录，以比较接种于各种培养基上的临床标本中棘阿米巴的回收率。然后我们比较了10株棘阿米巴角膜分离株在缓冲炭酵母提取物琼脂和血琼脂上的实验回收率。
在13例眼部棘阿米巴感染病例中进行的24次培养中，有缓冲炭酵母提取物琼脂和血琼脂的配对数据。在9次培养中，缓冲炭酵母提取物琼脂和血琼脂上均检测到棘阿米巴踪迹，9次培养仅在缓冲炭酵母提取物琼脂上检测到，1次培养仅在血琼脂上检测到（P = 0.027）。在实验研究中，所有10株临床分离株在缓冲炭酵母提取物琼脂上均产生踪迹，孵育10天后的平均回收率为原始接种量的38%至95%。10株分离株中有7株在缓冲炭酵母提取物琼脂上的回收率超过原始接种量的70%。10株菌株中只有2株在血琼脂上产生持续踪迹，孵育10天后的平均回收率分别为0.67%和1.17%。缓冲炭酵母提取物琼脂上的回收率明显高于血琼脂（P≤0.0003）。
缓冲炭酵母提取物琼脂是一种用于回收棘阿米巴的优良商用培养基。
