Hovhannisyan Vladimir, Ghazaryan Ara, Chen Yung-Fang, Chen Shean-Jen, Dong Chen-Yuan
Department of Physics, National Taiwan University, Taipei 106, Taiwan.
Opt Express. 2010 Nov 8;18(23):24037-47. doi: 10.1364/OE.18.024037.
Photophysical mechanisms of collagen photomodification (CFP) by the use of a 80 MHz, 780 nm femtosecond titanium-sapphire laser were investigated. Our observation that the decrease in collagen second harmonic generation and increase in two-photon autofluorescence intensity occurred primarily at sites where photoproducts were present suggested that the photoproducts may act to facilitate the CFP process. Laser power study of CFP indicated that the efficiency of the process depended on the sixth power of the laser intensity. Furthermore, it was demonstrated that CFP can be used for bending and cutting of collagen fibers and creating 3D patterns within collagen matrix with high precision (~2 μm).
研究了使用80MHz、780nm飞秒钛宝石激光对胶原蛋白进行光修饰（CFP）的光物理机制。我们观察到胶原蛋白二次谐波产生的减少和双光子自发荧光强度的增加主要发生在存在光产物的部位，这表明光产物可能有助于CFP过程。CFP的激光功率研究表明，该过程的效率取决于激光强度的六次方。此外，还证明了CFP可用于胶原蛋白纤维的弯曲和切割，并能在胶原蛋白基质内高精度（约2μm）地创建三维图案。
