Huang Yimei, Lui Harvey, Zhao Jianhua, Wu Zhenguo, Zeng Haishan
Imaging Unit - Integrative Oncology Department, BC Cancer Agency Research Center, Vancouver, BC, Canada.; Photomedicine Institute - Department of Dermatology and Skin Science, University of British Columbia and Vancouver Coastal Health Research Institute, Vancouver, BC, Canada.
Theranostics. 2017 Jan 6;7(3):513-522. doi: 10.7150/thno.17596. eCollection 2017.
The successful application of lasers in the treatment of skin diseases and cosmetic surgery is largely based on the principle of conventional selective photothermolysis which relies strongly on the difference in the absorption between the therapeutic target and its surroundings. However, when the differentiation in absorption is not sufficient, collateral damage would occur due to indiscriminate and nonspecific tissue heating. To deal with such cases, we introduce a novel method based on multiphoton absorption in which the radiant energy of a tightly focused near-infrared femtosecond laser beam can be directed spatially by aiming the laser focal point to the target of interest. We construct a multimodal optical microscope to perform and monitor the spatially selective photothermolysis. We demonstrate that precise alteration of the targeted tissue is achieved while leaving surrounding tissue intact by choosing appropriate femtosecond laser exposure with multimodal optical microscopy monitoring in real time.
激光在皮肤病治疗和整容手术中的成功应用很大程度上基于传统的选择性光热解原理，该原理强烈依赖于治疗靶点与其周围环境在吸收上的差异。然而，当吸收差异不足时，由于非特异性和无差别组织加热会发生附带损伤。为处理此类情况，我们引入一种基于多光子吸收的新方法，其中通过将激光焦点对准感兴趣的目标，可在空间上引导紧密聚焦的近红外飞秒激光束的辐射能。我们构建了一台多模态光学显微镜来执行和监测空间选择性光热解。我们证明，通过实时使用多模态光学显微镜监测选择合适的飞秒激光照射，可实现对目标组织的精确改变，同时使周围组织保持完整。
