Gualda Emilio J, Vázquez de Aldana Javier R, Martínez-García M Carmen, Moreno Pablo, Hernández-Toro Juan, Roso Luis, Artal Pablo, Bueno Juan M
Biomed Opt Express. 2011 Nov 1;2(11):2950-60. doi: 10.1364/BOE.2.002950. Epub 2011 Oct 3.
The performance of femtosecond (fs) laser intrastromal ablation was evaluated with backscattering-mode adaptive-optics multiphoton microscopy in ex vivo chicken corneas. The pulse energy of the fs source used for ablation was set to generate two different ablation patterns within the corneal stroma at a certain depth. Intrastromal patterns were imaged with a custom adaptive-optics multiphoton microscope to determine the accuracy of the procedure and verify the outcomes. This study demonstrates the potential of using fs pulses as surgical and monitoring techniques to systematically investigate intratissue ablation. Further refinement of the experimental system by combining both functions into a single fs laser system would be the basis to establish new techniques capable of monitoring corneal surgery without labeling in real-time. Since the backscattering configuration has also been optimized, future in vivo implementations would also be of interest in clinical environments involving corneal ablation procedures.
在离体鸡角膜中，利用背散射模式自适应光学多光子显微镜评估了飞秒（fs）激光基质内消融的性能。用于消融的飞秒光源的脉冲能量被设定为在角膜基质内的特定深度产生两种不同的消融模式。使用定制的自适应光学多光子显微镜对基质内模式进行成像，以确定该手术的准确性并验证结果。本研究证明了使用飞秒脉冲作为手术和监测技术来系统地研究组织内消融的潜力。通过将这两种功能整合到单个飞秒激光系统中对实验系统进行进一步优化，将成为建立能够实时无标记监测角膜手术的新技术的基础。由于背散射配置也已得到优化，未来在涉及角膜消融手术的临床环境中的体内应用也将备受关注。
